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Journal of Bacteriology, August 1999, p. 5111-5113, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Analysis of the rnc-era-recO Operon from Pseudomonas aeruginosa

Bradford Powell,1,dagger Howard K. Peters III,1 Yoshikazu Nakamura,2 and Donald Court1,*

Gene Regulation and Chromosome Biology Laboratory, ABL---Basic Research Program, Frederick Cancer Research and Development Center, National Cancer Institute, Frederick, Maryland 21702-12011, and Department of Tumor Biology, The Institute of Medical Science, The University of Tokyo, Takanawa 108, Japan2

Received 18 March 1999/Accepted 28 May 1999

The rnc operon from Pseudomonas aeruginosa has been cloned and characterized. The three genes comprising this operon, rnc, era, and recO, are arranged similarly to those in some other gram-negative bacteria. Multicopy plasmids carrying the rnc operon of P. aeruginosa functionally complement mutations of the rnc, era, and recO genes in Escherichia coli. In particular, the P. aeruginosa era homolog rescues the conditional lethality of era mutants in E. coli, and the presumptive protein has 60% identity with the Era of E. coli. We discuss these data and evidence suggesting that a GTPase previously purified from P. aeruginosa and designated Pra is not an Era homolog.

* Corresponding author. Mailing address: Gene Regulation and Chromosome Biology Laboratory, ABL---Basic Research Program, NCI---Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201. Phone: (301) 846-5940. Fax: (301) 846-6988. E-mail: court{at}ncifcrf.gov.

dagger Present address: Exponential Biotherapies, Inc., Rockville, MD 20850.

Journal of Bacteriology, August 1999, p. 5111-5113, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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