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Journal of Bacteriology, August 1999, p. 5119-5122, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning, Characterization, and Expression of a Novel Gene Encoding a Reversible 4-Hydroxybenzoate Decarboxylase from Clostridium hydroxybenzoicum

Jianbin Huang,dagger 2Hongqi He,Dagger and Juergen Wiegel*

Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602-2605

Received 1 March 1999/Accepted 2 June 1999

A novel gene, designated ohb1, which encodes the oxygen-sensitive and biotin-, ATP-, thiamin-, pyridoxal phosphate-, and metal-ion-independent, reversible 4-hydroxybenzoate decarboxylase (4-HOB-DC) from the obligate anaerobe Clostridium hydroxybenzoicum JW/Z-1T was sequenced (GenBank accession no. AF128880) and expressed. The 1,440-bp open reading frame (ORF) (ohb1) encodes 480 amino acids. Major properties of the heterologous enzyme (Ohb1) expressed in Escherichia coli DH5alpha were the same as those described for the native 4-HOB-DC (Z. He and J. Wiegel, J. Bacteriol. 178:3539-3543, 1996). The deduced amino acid sequence shows up to 57% identity and up to 74% similarity to hypothetical proteins deduced from ORFs in genomes from bacteria and archaea, suggesting a possible novel gene family.

* Corresponding author. Mailing address: University of Georgia, Department of Microbiology, 215 Biological Science Building, Athens, GA 30602-2605. Phone: (706) 542-2651. Fax: (706) 542-2674. E-mail: JWIEGEL{at}arches.uga.edu.

dagger Present address: Department of Biochemistry Center for Biomolecular Structure Analysis, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284.

Dagger Present address: Air Force Research Lab, Tyndall Air Force Base, FL 32403-5323.

Journal of Bacteriology, August 1999, p. 5119-5122, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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