This Article Full Text Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, J. Articles by Wiegel, J. Search for Related Content PubMed PubMed Citation Articles by Huang, J. Articles by Wiegel, J.

Journal of Bacteriology, August 1999, p. 5119-5122, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning, Characterization, and Expression of a Novel Gene Encoding a Reversible 4-Hydroxybenzoate Decarboxylase from Clostridium hydroxybenzoicum

Jianbin Huang, 2Hongqi He, and Juergen Wiegel^*

Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602-2605

Received 1 March 1999/Accepted 2 June 1999

A novel gene, designated ohb1, which encodes the oxygen-sensitive and biotin-, ATP-, thiamin-, pyridoxal phosphate-, and metal-ion-independent, reversible 4-hydroxybenzoate decarboxylase (4-HOB-DC) from the obligate anaerobe Clostridium hydroxybenzoicum JW/Z-1^T was sequenced (GenBank accession no. AF128880) and expressed. The 1,440-bp open reading frame (ORF) (ohb1) encodes 480 amino acids. Major properties of the heterologous enzyme (Ohb1) expressed in Escherichia coli DH5 were the same as those described for the native 4-HOB-DC (Z. He and J. Wiegel, J. Bacteriol. 178:3539-3543, 1996). The deduced amino acid sequence shows up to 57% identity and up to 74% similarity to hypothetical proteins deduced from ORFs in genomes from bacteria and archaea, suggesting a possible novel gene family.

---

^* Corresponding author. Mailing address: University of Georgia, Department of Microbiology, 215 Biological Science Building, Athens, GA 30602-2605. Phone: (706) 542-2651. Fax: (706) 542-2674. E-mail: JWIEGEL{at}arches.uga.edu.

Present address: Department of Biochemistry Center for Biomolecular Structure Analysis, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284.

Present address: Air Force Research Lab, Tyndall Air Force Base, FL 32403-5323.

---

Journal of Bacteriology, August 1999, p. 5119-5122, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• FUCHS, G. (2008). Anaerobic Metabolism of Aromatic Compounds. Ann. N. Y. Acad. Sci. 1125: 82-99 [Abstract] [Full Text]  
• Goto, M., Hayashi, H., Miyahara, I., Hirotsu, K., Yoshida, M., Oikawa, T. (2006). Crystal Structures of Nonoxidative Zinc-dependent 2,6-Dihydroxybenzoate ({gamma}-Resorcylate) Decarboxylase from Rhizobium sp. Strain MTP-10005. J. Biol. Chem. 281: 34365-34373 [Abstract] [Full Text]  
• Peng, X., Masai, E., Kasai, D., Miyauchi, K., Katayama, Y., Fukuda, M. (2005). A Second 5-Carboxyvanillate Decarboxylase Gene, ligW2, Is Important for Lignin-Related Biphenyl Catabolism in Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 71: 5014-5021 [Abstract] [Full Text]  
• Yoshida, M., Fukuhara, N., Oikawa, T. (2004). Thermophilic, Reversible {gamma}-Resorcylate Decarboxylase from Rhizobium sp. Strain MTP-10005: Purification, Molecular Characterization, and Expression. J. Bacteriol. 186: 6855-6863 [Abstract] [Full Text]  
• Schuhle, K., Fuchs, G. (2004). Phenylphosphate Carboxylase: a New C-C Lyase Involved in Anaerobic Phenol Metabolism in Thauera aromatica. J. Bacteriol. 186: 4556-4567 [Abstract] [Full Text]  
• Breinig, S., Schiltz, E., Fuchs, G. (2000). Genes Involved in Anaerobic Metabolism of Phenol in the Bacterium Thauera aromatica. J. Bacteriol. 182: 5849-5863 [Abstract] [Full Text]