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Journal of Bacteriology, August 1999, p. 5126-5130, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

DsbA Is Required for Stable Expression of Outer Membrane Protein YscC and for Efficient Yop Secretion in Yersinia pestis

Michael W. Jackson and Gregory V. Plano*

Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida 33176

Received 11 March 1999/Accepted 10 June 1999

The role of the periplasmic disulfide oxidoreductase DsbA in Yop secretion was investigated in Yersinia pestis. A Y. pestis dsbA mutant secreted reduced amounts of the V antigen and Yops and expressed reduced amounts of the full-sized YscC protein. Site-directed mutagenesis of the four cysteine residues present in the YscC protein resulted in defects similar to those found in the dsbA mutant. These results suggest that YscC contains at least one disulfide bond that is essential for the function of this protein in Yop secretion.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, FL 33176. Phone: (305) 243-6310. Fax: (305) 243-4623. E-mail: gplano{at}mednet.med.miami.edu.

Journal of Bacteriology, August 1999, p. 5126-5130, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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