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Journal of Bacteriology, August 1999, p. 5131-5133, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Ligand Binding of EmrR, a Regulator of a Multidrug Transporter

Alexei Brooun, John J. Tomashek, and Kim Lewis*

Biotechnology Center, Tufts University, Medford, Massachusetts 02155

Received 10 May 1999/Accepted 8 June 1999

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump---2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone---with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR.

* Corresponding author. Mailing address: Biotechnology Center, Tufts University, 4 Colby St., Medford, MA 02155. Phone: (617) 627-3731. Fax: (617) 627-3993. E-mail: klewis{at}tufts.edu.

Journal of Bacteriology, August 1999, p. 5131-5133, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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