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Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Combined Genetic and Physical Map of the Complex Genome of Agrobacterium tumefaciens

Brad W. Goodner,* Brian P. Markelz, M. Casey Flanagan, Chris B. Crowell Jr., Jodi L. Racette, Brittany A. Schilling, Leah M. Halfon, J. Scott Mellors, and Gregory Grabowski

Department of Biology, University of Richmond, Richmond, Virginia 23173

Received 5 March 1999/Accepted 10 June 1999

A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.

* Corresponding author. Mailing address: Department of Biology, Science Center E-105, University of Richmond, Richmond, VA 23173. Phone: (804) 289-8661. Fax: (804) 289-8233. E-mail: bgoodner{at}richmond.edu.

Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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