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Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Combined Genetic and Physical Map of the Complex
Genome of Agrobacterium tumefaciens
Brad W.
Goodner,*
Brian P.
Markelz,
M. Casey
Flanagan,
Chris B.
Crowell Jr.,
Jodi L.
Racette,
Brittany A.
Schilling,
Leah M.
Halfon,
J. Scott
Mellors, and
Gregory
Grabowski
Department of Biology, University of
Richmond, Richmond, Virginia 23173
Received 5 March 1999/Accepted 10 June 1999
A combined genetic and physical map of the Agrobacterium
tumefaciens A348 (derivative of C58) genome was constructed to
address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two
nonhomologous chromosomes. The combined map confirms the two-chromosome
genomic structure and the correspondence of the initial genetic maps to
the circular chromosome. The linear chromosome is almost devoid of
auxotrophic markers, which probably explains why it was missed by
genetic mapping studies.
*
Corresponding author. Mailing address: Department of
Biology, Science Center E-105, University of Richmond, Richmond, VA
23173. Phone: (804) 289-8661. Fax: (804) 289-8233. E-mail:
bgoodner{at}richmond.edu.
Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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