This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goodner, B. W.
Right arrow Articles by Grabowski, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goodner, B. W.
Right arrow Articles by Grabowski, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Combined Genetic and Physical Map of the Complex Genome of Agrobacterium tumefaciens

Brad W. Goodner,* Brian P. Markelz, M. Casey Flanagan, Chris B. Crowell Jr., Jodi L. Racette, Brittany A. Schilling, Leah M. Halfon, J. Scott Mellors, and Gregory Grabowski

Department of Biology, University of Richmond, Richmond, Virginia 23173

Received 5 March 1999/Accepted 10 June 1999

A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.

* Corresponding author. Mailing address: Department of Biology, Science Center E-105, University of Richmond, Richmond, VA 23173. Phone: (804) 289-8661. Fax: (804) 289-8233. E-mail: bgoodner{at}richmond.edu.

Journal of Bacteriology, September 1999, p. 5160-5166, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Marri, P. R., Harris, L. K., Houmiel, K., Slater, S. C., Ochman, H. (2008). The Effect of Chromosome Geometry on Genetic Diversity. Genetics 179: 511-516 [Abstract] [Full Text]  
  • Jurkowski, A., Reid, A. H., Labov, J. B. (2007). Metagenomics: A Call for Bringing a New Science into the Classroom (While It's Still New). cellbioed 6: 260-265 [Full Text]  
  • De Costa, D. M., Suzuki, K., Yoshida, K. (2003). Structural and Functional Analysis of a Putative Gene Cluster for Palatinose Transport on the Linear Chromosome of Agrobacterium tumefaciens MAFF301001. J. Bacteriol. 185: 2369-2373 [Abstract] [Full Text]  
  • Gelvin, S. B. (2003). Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool. Microbiol. Mol. Biol. Rev. 67: 16-37 [Abstract] [Full Text]  
  • Rogers, E. J., Rahman, M. S., Hill, R. T., Lovett, P. S. (2002). The Chloramphenicol-Inducible catB Gene in Agrobacterium tumefaciens Is Regulated by Translation Attenuation. J. Bacteriol. 184: 4296-4300 [Abstract] [Full Text]  
  • Goodner, B., Hinkle, G., Gattung, S., Miller, N., Blanchard, M., Qurollo, B., Goldman, B. S., Cao, Y., Askenazi, M., Halling, C., Mullin, L., Houmiel, K., Gordon, J., Vaudin, M., Iartchouk, O., Epp, A., Liu, F., Wollam, C., Allinger, M., Doughty, D., Scott, C., Lappas, C., Markelz, B., Flanagan, C., Crowell, C., Gurson, J., Lomo, C., Sear, C., Strub, G., Cielo, C., Slater, S. (2001). Genome Sequence of the Plant Pathogen and Biotechnology Agent Agrobacterium tumefaciens C58. Science 294: 2323-2328 [Abstract] [Full Text]  
  • Kahng, L. S., Shapiro, L. (2001). The CcrM DNA Methyltransferase of Agrobacterium tumefaciens Is Essential, and Its Activity Is Cell Cycle Regulated. J. Bacteriol. 183: 3065-3075 [Abstract] [Full Text]  
  • Parke, D. (2000). Positive Selection for Mutations Affecting Bioconversion of Aromatic Compounds in Agrobacterium tumefaciens: Analysis of Spontaneous Mutations in the Protocatechuate 3,4-Dioxygenase Gene. J. Bacteriol. 182: 6145-6153 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»