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Journal of Bacteriology, September 1999, p. 5167-5175, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Timing of FtsZ Assembly in Escherichia coli

Tanneke Den Blaauwen,* Nienke Buddelmeijer,dagger Mirjam E. G. Aarsman, Cor M. Hameete, and Nanne Nanninga

Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam, The Netherlands

Received 18 March 1999/Accepted 16 June 1999

The timing of the appearance of the FtsZ ring at the future site of division in Escherichia coli was determined by in situ immunofluorescence microscopy for two strains grown under steady-state conditions. The strains, B/rA and K-12 MC4100, differ largely in the duration of the D period, the time between termination of DNA replication and cell division. In both strains and under various growth conditions, the assembly of the FtsZ ring was initiated approximately simultaneously with the start of the D period. This is well before nucleoid separation or initiation of constriction as determined by fluorescence and phase-contrast microscopy. The durations of the Z-ring period, the D period, and the period with a visible constriction seem to be correlated under all investigated growth conditions in these strains. These results suggest that (near) termination of DNA replication could provide a signal that initiates the process of cell division.

* Corresponding author. Mailing address: Institute for Molecular Cell Biology, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. Phone: 31-20-5255196/5187. Fax: 31-20-5256271. E-mail: blaauwen{at}bio.uva.nl.

dagger Present address: Department of Microbiology, Harvard Medical School, Boston, MA 02115.

Journal of Bacteriology, September 1999, p. 5167-5175, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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