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Journal of Bacteriology, September 1999, p. 5257-5262, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Suppression of Nonsense Mutations Induced by Expression of an RNA Complementary to a Conserved Segment of 23S rRNA

Natalya S. Chernyaeva,1,2,dagger Emanuel J. Murgola,2 and Alexander S. Mankin1,*

Center for Pharmaceutical Biotechnology, The University of Illinois, Chicago, Illinois 60607,1 and Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 770302

Received 29 April 1999/Accepted 28 June 1999

We identified a short RNA fragment, complementary to the Escherichia coli 23S rRNA segment comprising nucleotides 735 to 766 (in domain II), which when expressed in vivo results in the suppression of UGA nonsense mutations in two reporter genes. Neither UAA nor UAG mutations, examined at the same codon positions, were suppressed by the expression of this antisense rRNA fragment. Our results suggest that a stable phylogenetically conserved hairpin at nucleotides 736 to 760 in 23S rRNA, which is situated close to the peptidyl transferase center, may participate in one or more specific interactions during peptide chain termination.

* Corresponding author. Mailing address: Center for Pharmaceutical Biotechnology---m/c 870, Room 3052, University of Illinois, 900 S. Ashland Ave., Chicago, IL 60607. Phone: (312) 413-1406. Fax: (312) 413-9303. E-mail: shura{at}uic.edu.

dagger Present address: Department of Molecular Genetics, M. D. Anderson Cancer Center, Houston, TX 77030.

Journal of Bacteriology, September 1999, p. 5257-5262, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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