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Journal of Bacteriology, September 1999, p. 5384-5388, Vol. 181, No. 17
0021-9193/99/$04.00+0

Role of the Sporulation Protein BofA in Regulating Activation of the Bacillus subtilis Developmental Transcription Factor ^K

Orna Resnekov^*

Section on Microbial Genetics, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2785

Received 1 March 1999/Accepted 21 June 1999

During sporulation, the Bacillus subtilis transcription factor ^K is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro-^K to ^K. The results show that in the presence of BofA, SpoIVFA levels increase by greater than 20-fold, SpoIVFA is substantially stabilized, and pro-^K processing is inhibited. In addition, enhanced accumulation of the SpoIVFA protein in the absence of BofA (achieved through the use of an ftsH null mutation) substantially inhibits pro-^K processing. These results suggest that during growth, increased accumulation of the SpoIVFA protein inhibits the activity of SpoIVFB-GFP and regulates the activation of ^K.

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^* Mailing address: NICHD-LMG, Building 6B-304, National Institutes of Health, 6 Center Dr., MSC 2785, Bethesda, MD 20892-2785. Phone: (301) 496-5663. Fax: (301) 496-0243. E-mail: resnekov{at}biosun.harvard.edu.

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Journal of Bacteriology, September 1999, p. 5384-5388, Vol. 181, No. 17
0021-9193/99/$04.00+0

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