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Journal of Bacteriology, September 1999, p. 5516-5520, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg2+-Responsive Promoters

Akinori Kato, Hiroyuki Tanabe, and Ryutaro Utsumi*

Department of Agricultural Chemistry, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan

Received 11 March 1999/Accepted 21 June 1999

We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

* Corresponding author. Mailing address: Department of Agricultural Chemistry, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan. Phone: 81-742-43-151. Fax: 81-742-43-1445. E-mail: utsumi{at}nara.kindai.ac.jp.

Journal of Bacteriology, September 1999, p. 5516-5520, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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