This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hols, P.
Right arrow Articles by Kleerebezem, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hols, P.
Right arrow Articles by Kleerebezem, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1999, p. 5521-5526, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Acetate Utilization in Lactococcus lactis Deficient in Lactate Dehydrogenase: a Rescue Pathway for Maintaining Redox Balance

Pascal Hols,1,2,* Ana Ramos,3 Jeroen Hugenholtz,1 Jean Delcour,2 Willem M. de Vos,1 Helena Santos,3 and Michiel Kleerebezem1

Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands1; Laboratoire de Génétique Moléculaire, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium2; and Instituto de Biologia Experimental e Tecnológica, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780 Oeiras, Portugal3

Received 8 March 1999/Accepted 27 June 1999

Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase. 13C and 1H nuclear magnetic resonance studies using [2-13C]glucose and [2-13C]acetate as substrates demonstrated that acetate was exclusively converted to ethanol. This novel pathway provides an alternative route for NAD+ regeneration in the absence of lactate dehydrogenase.

* Corresponding author. Mailing address: Laboratoire de Génétique Moléculaire, Université catholique de Louvain, 5 Place Croix du Sud, B-1348 Louvain-La-Neuve, Belgium. Phone: 32-10-478896. Fax: 32-10-473109. E-mail: hols{at}gene.ucl.ac.be.

Journal of Bacteriology, September 1999, p. 5521-5526, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ladero, V., Ramos, A., Wiersma, A., Goffin, P., Schanck, A., Kleerebezem, M., Hugenholtz, J., Smid, E. J., Hols, P. (2007). High-Level Production of the Low-Calorie Sugar Sorbitol by Lactobacillus plantarum through Metabolic Engineering. Appl. Environ. Microbiol. 73: 1864-1872 [Abstract] [Full Text]  
  • Bongers, R. S., Hoefnagel, M. H. N., Kleerebezem, M. (2005). High-Level Acetaldehyde Production in Lactococcus lactis by Metabolic Engineering. Appl. Environ. Microbiol. 71: 1109-1113 [Abstract] [Full Text]  
  • Gaspar, P., Neves, A. R., Ramos, A., Gasson, M. J., Shearman, C. A., Santos, H. (2004). Engineering Lactococcus lactis for Production of Mannitol: High Yields from Food-Grade Strains Deficient in Lactate Dehydrogenase and the Mannitol Transport System. Appl. Environ. Microbiol. 70: 1466-1474 [Abstract] [Full Text]  
  • Bongers, R. S., Hoefnagel, M. H. N., Starrenburg, M. J. C., Siemerink, M. A. J., Arends, J. G. A., Hugenholtz, J., Kleerebezem, M. (2003). IS981-Mediated Adaptive Evolution Recovers Lactate Production by ldhB Transcription Activation in a Lactate Dehydrogenase-Deficient Strain of Lactococcus lactis. J. Bacteriol. 185: 4499-4507 [Abstract] [Full Text]  
  • Nordkvist, M., Jensen, N. B. S., Villadsen, J. (2003). Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions. Appl. Environ. Microbiol. 69: 3462-3468 [Abstract] [Full Text]  
  • Neves, A. R., Ramos, A., Costa, H., van Swam, I. I., Hugenholtz, J., Kleerebezem, M., de Vos, W., Santos, H. (2002). Effect of Different NADH Oxidase Levels on Glucose Metabolism by Lactococcus lactis: Kinetics of Intracellular Metabolite Pools Determined by In Vivo Nuclear Magnetic Resonance. Appl. Environ. Microbiol. 68: 6332-6342 [Abstract] [Full Text]  
  • Lynd, L. R., Weimer, P. J., van Zyl, W. H., Pretorius, I. S. (2002). Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577 [Abstract] [Full Text]  
  • Neves, A. R., Ventura, R., Mansour, N., Shearman, C., Gasson, M. J., Maycock, C., Ramos, A., Santos, H. (2002). Is the Glycolytic Flux in Lactococcus lactis Primarily Controlled by the Redox Charge? KINETICS OF NAD+ AND NADH POOLS DETERMINED IN VIVO BY 13C NMR. J. Biol. Chem. 277: 28088-28098 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»