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Journal of Bacteriology, September 1999, p. 5521-5526, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Acetate Utilization in Lactococcus lactis Deficient in Lactate Dehydrogenase: a Rescue Pathway for Maintaining Redox Balance

Pascal Hols,1,2,* Ana Ramos,3 Jeroen Hugenholtz,1 Jean Delcour,2 Willem M. de Vos,1 Helena Santos,3 and Michiel Kleerebezem1

Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands1; Laboratoire de Génétique Moléculaire, Université catholique de Louvain, B-1348 Louvain-La-Neuve, Belgium2; and Instituto de Biologia Experimental e Tecnológica, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780 Oeiras, Portugal3

Received 8 March 1999/Accepted 27 June 1999

Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase. 13C and 1H nuclear magnetic resonance studies using [2-13C]glucose and [2-13C]acetate as substrates demonstrated that acetate was exclusively converted to ethanol. This novel pathway provides an alternative route for NAD+ regeneration in the absence of lactate dehydrogenase.


* Corresponding author. Mailing address: Laboratoire de Génétique Moléculaire, Université catholique de Louvain, 5 Place Croix du Sud, B-1348 Louvain-La-Neuve, Belgium. Phone: 32-10-478896. Fax: 32-10-473109. E-mail: hols{at}gene.ucl.ac.be.


Journal of Bacteriology, September 1999, p. 5521-5526, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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