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Journal of Bacteriology, September 1999, p. 5527-5529, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Clustering of the Chemoreceptor Complex in Escherichia coli Is Independent of the Methyltransferase CheR and the Methylesterase CheB

Suzanne R. Lybarger and Janine R. Maddock*

Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048

Received 26 April 1999/Accepted 22 June 1999

The Escherichia coli chemoreceptors and their associated cytoplasmic proteins, CheA and CheW, cluster predominantly at the cell poles. The nature of the clustering remains a mystery. Recent studies suggest that CheR binding to and/or methylation of the chemoreceptors may play a role in chemoreceptor complex aggregation. In this study, we examined the intracellular distribution of the chemoreceptors by immunoelectron microscopy in strains lacking either the methyltransferase CheR or the methylesterase CheB. The localization data revealed that, in vivo, aggregation of the chemoreceptor complex was independent of either CheR or CheB.

* Corresponding author. Mailing address: Department of Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109-1048. Phone: (734) 936-8068. Fax: (734) 647-0884. E-mail: maddock{at}biology.lsa.umich.edu.

Journal of Bacteriology, September 1999, p. 5527-5529, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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