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Journal of Bacteriology, September 1999, p. 5534-5538, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of a Conserved N-Terminal Sequence Involved in Transmembrane Signal Transduction in EnvZ

Jill Waukau and Steven Forst*

Department of Biological Sciences, University of Wisconsin, Milwaukee, Wisconsin 53201

Received 20 April 1999/Accepted 17 June 1999

To determine whether N-terminal sequences are involved in the transmembrane signaling mechanism of EnvZ, the nucleotide sequences of envZ genes from several enteric bacteria were determined. Comparative analysis revealed that the amino acid sequence between Pro41 and Glu53 was highly conserved. To further analyze the role of the conserved sequence, envZ of Escherichia coli was subjected to random PCR mutagenesis and mutant alleles that produced a high-osmolarity phenotype, in which ompF was repressed, were isolated. The mutations identified clustered within, as well as adjacent to, the Pro41-to-Glu53 sequence. These findings suggest that the conserved Pro41-to-Glu53 sequence is involved in the signal transduction mechanism of EnvZ.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Wisconsin, P.O. Box 413, Milwaukee, WI 53201. Phone: (414) 229-6373. Fax: (414) 229-3926. E-mail: sforst{at}csd.uwm.edu.

Journal of Bacteriology, September 1999, p. 5534-5538, Vol. 181, No. 17
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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