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Journal of Bacteriology, September 1999, p. 5591-5599, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Diversity and Mosaicism at the por Locus of Neisseria gonorrhoeae

Trevor C. Fudyk,1 Ian W. Maclean,1 J. Neil Simonsen,1 Ephantus N. Njagi,2 Joshua Kimani,2 Robert C. Brunham,1 and Francis A. Plummer1,2,*

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada,1 and Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya2

Received 19 January 1999/Accepted 18 June 1999

The por genes of the predominant serovars of Neisseria gonorrhoeae circulating in a high-frequency transmitter core group located in Nairobi, Kenya, were examined for nucleotide sequence polymorphism. The level of por gene diversity did not differ significantly between core group-derived gonococcal strains and gonococcal strains originating elsewhere. However, por mosaicism appeared to be more frequent among core group-derived strains, suggesting that recombination of different por sequences may be a important strategy by which N. gonorrhoeae generates por gene diversity within core group populations. Despite extensive sequence variability, por expressed by gonococcal isolates of different geographic origin exhibited conserved patterns of nucleotide change, suggesting that diversity among por alleles may also be finite.

* Corresponding author. Mailing address: Department of Medical Microbiology, Room 514, Basic Medical Sciences Building, University of Manitoba, 730 William Ave., Winnipeg, Manitoba, Canada R3E 0W3. Phone: (204) 789-3310. Fax: (204) 789-3926. E-mail: plummer{at}form-net.com.

Journal of Bacteriology, September 1999, p. 5591-5599, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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