This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fudyk, T. C.
Right arrow Articles by Plummer, F. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fudyk, T. C.
Right arrow Articles by Plummer, F. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1999, p. 5591-5599, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Diversity and Mosaicism at the por Locus of Neisseria gonorrhoeae

Trevor C. Fudyk,1 Ian W. Maclean,1 J. Neil Simonsen,1 Ephantus N. Njagi,2 Joshua Kimani,2 Robert C. Brunham,1 and Francis A. Plummer1,2,*

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada,1 and Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya2

Received 19 January 1999/Accepted 18 June 1999

The por genes of the predominant serovars of Neisseria gonorrhoeae circulating in a high-frequency transmitter core group located in Nairobi, Kenya, were examined for nucleotide sequence polymorphism. The level of por gene diversity did not differ significantly between core group-derived gonococcal strains and gonococcal strains originating elsewhere. However, por mosaicism appeared to be more frequent among core group-derived strains, suggesting that recombination of different por sequences may be a important strategy by which N. gonorrhoeae generates por gene diversity within core group populations. Despite extensive sequence variability, por expressed by gonococcal isolates of different geographic origin exhibited conserved patterns of nucleotide change, suggesting that diversity among por alleles may also be finite.

* Corresponding author. Mailing address: Department of Medical Microbiology, Room 514, Basic Medical Sciences Building, University of Manitoba, 730 William Ave., Winnipeg, Manitoba, Canada R3E 0W3. Phone: (204) 789-3310. Fax: (204) 789-3926. E-mail: plummer{at}form-net.com.

Journal of Bacteriology, September 1999, p. 5591-5599, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ilina, E. N., Borovskaya, A. D., Malakhova, M. M., Vereshchagin, V. A., Kubanova, A. A., Kruglov, A. N., Svistunova, T. S., Gazarian, A. O., Maier, T., Kostrzewa, M., Govorun, V. M. (2009). Direct Bacterial Profiling by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry for Identification of Pathogenic Neisseria. J. Mol. Diagn. 11: 75-86 [Abstract] [Full Text]  
  • Tsuru, T., Kobayashi, I. (2008). Multiple Genome Comparison within a Bacterial Species Reveals a Unit of Evolution Spanning Two Adjacent Genes in a Tandem Paralog Cluster. Mol Biol Evol 25: 2457-2473 [Abstract] [Full Text]  
  • Garvin, L. E., Bash, M. C., Keys, C., Warner, D. M., Ram, S., Shafer, W. M., Jerse, A. E. (2008). Phenotypic and Genotypic Analyses of Neisseria gonorrhoeae Isolates That Express Frequently Recovered PorB PIA Variable Region Types Suggest that Certain P1a Porin Sequences Confer a Selective Advantage for Urogenital Tract Infection. Infect. Immun. 76: 3700-3709 [Abstract] [Full Text]  
  • Ilina, E. N., Vereshchagin, V. A., Borovskaya, A. D., Malakhova, M. V., Sidorenko, S. V., Al-Khafaji, N. C., Kubanova, A. A., Govorun, V. M. (2008). Relation between Genetic Markers of Drug Resistance and Susceptibility Profile of Clinical Neisseria gonorrhoeae Strains. Antimicrob. Agents Chemother. 52: 2175-2182 [Abstract] [Full Text]  
  • Liao, M., Bell, K., Gu, W.-M., Yang, Y., Eng, N. F., Fu, W., Wu, L., Zhang, C.-G., Chen, Y., Jolly, A. M., Dillon, J.-A. R. (2008). Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai. J Antimicrob Chemother 61: 478-487 [Abstract] [Full Text]  
  • Hansen, J. K., Demick, K. P., Mansfield, J. M., Forest, K. T. (2007). Conserved Regions from Neisseria gonorrhoeae Pilin Are Immunosilent and Not Immunosuppressive. Infect. Immun. 75: 4138-4147 [Abstract] [Full Text]  
  • Takahata, S., Senju, N., Osaki, Y., Yoshida, T., Ida, T. (2006). Amino Acid Substitutions in Mosaic Penicillin-Binding Protein 2 Associated with Reduced Susceptibility to Cefixime in Clinical Isolates of Neisseria gonorrhoeae. Antimicrob. Agents Chemother. 50: 3638-3645 [Abstract] [Full Text]  
  • Perez-Losada, M., Viscidi, R. P., Demma, J. C., Zenilman, J., Crandall, K. A. (2005). Population Genetics of Neisseria gonorrhoeae in a High-Prevalence Community Using a Hypervariable Outer Membrane porB and 13 Slowly Evolving Housekeeping Genes. Mol Biol Evol 22: 1887-1902 [Abstract] [Full Text]  
  • Bash, M. C., Zhu, P., Gulati, S., McKnew, D., Rice, P. A., Lynn, F. (2005). por Variable-Region Typing by DNA Probe Hybridization Is Broadly Applicable to Epidemiologic Studies of Neisseria gonorrhoeae. J. Clin. Microbiol. 43: 1522-1530 [Abstract] [Full Text]  
  • Lynn, F., Hobbs, M. M., Zenilman, J. M., Behets, F. M. T. F., Van Damme, K., Rasamindrakotroka, A., Bash, M. C. (2005). Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections. J. Clin. Microbiol. 43: 368-375 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Jonasson, J., Fredlund, H. (2004). Molecular Typing of Neisseria gonorrhoeae Isolates by Pyrosequencing of Highly Polymorphic Segments of the porB Gene. J. Clin. Microbiol. 42: 2926-2934 [Abstract] [Full Text]  
  • Martin, I. M. C., Ghani, A., Bell, G., Kinghorn, G., Ison, C. A. (2003). Persistence of Two Genotypes of Neisseria gonorrhoeae during Transmission. J. Clin. Microbiol. 41: 5609-5614 [Abstract] [Full Text]  
  • Polley, S. D., Chokejindachai, W., Conway, D. J. (2003). Allele Frequency-Based Analyses Robustly Map Sequence Sites Under Balancing Selection in a Malaria Vaccine Candidate Antigen. Genetics 165: 555-561 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Albert, J., Fredlund, H. (2003). Comparison of Serologic and Genetic porB-Based Typing of Neisseria gonorrhoeae: Consequences for Future Characterization. J. Clin. Microbiol. 41: 4141-4147 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Berglund, T., Albert, J., Fredlund, H. (2002). Molecular Epidemiology of Neisseria gonorrhoeae: Sequence Analysis of the porB Gene Confirms Presence of Two Circulating Strains. J. Clin. Microbiol. 40: 3741-3749 [Abstract] [Full Text]  
  • Olesky, M., Hobbs, M., Nicholas, R. A. (2002). Identification and Analysis of Amino Acid Mutations in Porin IB That Mediate Intermediate-Level Resistance to Penicillin and Tetracycline in Neisseria gonorrhoeae. Antimicrob. Agents Chemother. 46: 2811-2820 [Abstract] [Full Text]  
  • Nesbo, C. L., Nelson, K. E., Doolittle, W. F. (2002). Suppressive Subtractive Hybridization Detects Extensive Genomic Diversity in Thermotoga maritima. J. Bacteriol. 184: 4475-4488 [Abstract] [Full Text]  
  • Shafer, W. M., Datta, A., Kumar Kolli, V.S., Mahbubur Rahman, M., Balthazar, J. T., Martin, L. E., Veal, W. L., Stephens, D. S., Carlson, R. (2002). Phase variable changes in genes lgtA and lgtC within the lgtABCDE operon of Neisseria gonorrhoeae can modulate gonococcal susceptibility to normal human serum. Innate Immunity 8: 47-58 [Abstract]  
  • Sumie, A., Yamashiro, T., Nakashima, K., Nasu, M., Watanabe, M., Nishizono, A. (2001). Comparison of Genomic Structures and Antigenic Reactivities of Orthologous 29-Kilodalton Outer Membrane Proteins of Helicobacter pylori. Infect. Immun. 69: 6846-6852 [Abstract] [Full Text]  
  • Paquet, J.-Y., Diaz, M. A., Genevrois, S., Grayon, M., Verger, J.-M., De Bolle, X., Lakey, J. H., Letesson, J.-J., Cloeckaert, A. (2001). Molecular, Antigenic, and Functional Analyses of Omp2b Porin Size Variants of Brucella spp.. J. Bacteriol. 183: 4839-4847 [Abstract] [Full Text]  
  • Nesb, C. L., L'Haridon, S., Stetter, K. O., Doolittle, W. F. (2001). Phylogenetic Analyses of Two "Archaeal" Genes in Thermotoga maritima Reveal Multiple Transfers Between Archaea and Bacteria. Mol Biol Evol 18: 362-375 [Abstract] [Full Text]  
  • Ram, S., Cullinane, M., Blom, A. M., Gulati, S., McQuillen, D. P., Monks, B. G., O'Connell, C., Boden, R., Elkins, C., Pangburn, M. K., Dahlback, B., Rice, P. A. (2001). Binding of C4b-Binding Protein to Porin: A Molecular Mechanism of Serum Resistance of Neisseria gonorrhoeae. JEM 193: 281-296 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»