This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Junker, F.
Right arrow Articles by Ramos, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Junker, F.
Right arrow Articles by Ramos, J. L.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 1999, p. 5693-5700, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Involvement of the cis/trans Isomerase Cti in Solvent Resistance of Pseudomonas putida DOT-T1E

Frank Junker and Juan L. Ramos*

Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, E-18008 Granada, Spain

Received 8 February 1999/Accepted 23 June 1999

Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholipids, mainly cis-oleic acid (C16:1,9) and cis-vaccenic acid (C18:1,11), in response to solvents. To determine the importance of this cis/trans isomerase for solvent resistance a Cti-null mutant was generated and characterized. This mutant showed a longer lag phase when grown with toluene in the vapor phase; however, after the lag phase the growth rate of the mutant strain was similar to that of the wild type. The mutant also showed a significantly lower survival rate when shocked with 0.08% (vol/vol) toluene. In contrast to the wild-type strain, which grew in liquid culture medium at temperatures up to 38.5°C, the Cti-null mutant strain grew significantly slower at temperatures above 37°C. An in-frame fusion of the Cti protein with the periplasmic alkaline phosphatase suggests that this constitutively expressed enzyme is located in the periplasm. Primer extension studies confirmed the constitutive expression of Cti. Southern blot analysis of total DNA from various pseudomonads showed that the cti gene is present in all the tested P. putida strains, including non-solvent-resistant ones, and in some other Pseudomonas species.

* Corresponding author. Mailing address: CSIC-Estacion Experimental del Zaidin, Profesor Albareda 1, E-18008 Granada, Spain. Phone: 34-958-121011. Fax: 34-958-129600. E-mail: jlramos{at}eez.csic.es.

Journal of Bacteriology, September 1999, p. 5693-5700, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nikodinovic-Runic, J., Flanagan, M., Hume, A. R., Cagney, G., O'Connor, K. E. (2009). Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions. Microbiology 155: 3348-3361 [Abstract] [Full Text]  
  • Bonner, P. J., Shimkets, L. J. (2006). Cohesion-Defective Mutants of Myxococcus xanthus.. J. Bacteriol. 188: 4585-4588 [Abstract] [Full Text]  
  • Dominguez-Cuevas, P., Gonzalez-Pastor, J.-E., Marques, S., Ramos, J.-L., de Lorenzo, V. (2006). Transcriptional Tradeoff between Metabolic and Stress-response Programs in Pseudomonas putida KT2440 Cells Exposed to Toluene. J. Biol. Chem. 281: 11981-11991 [Abstract] [Full Text]  
  • Neumann, G., Kabelitz, N., Zehnsdorf, A., Miltner, A., Lippold, H., Meyer, D., Schmid, A., Heipieper, H. J. (2005). Prediction of the Adaptability of Pseudomonas putida DOT-T1E to a Second Phase of a Solvent for Economically Sound Two-Phase Biotransformations. Appl. Environ. Microbiol. 71: 6606-6612 [Abstract] [Full Text]  
  • Nielsen, L. E., Kadavy, D. R., Rajagopal, S., Drijber, R., Nickerson, K. W. (2005). Survey of Extreme Solvent Tolerance in Gram-Positive Cocci: Membrane Fatty Acid Changes in Staphylococcus haemolyticus Grown in Toluene. Appl. Environ. Microbiol. 71: 5171-5176 [Abstract] [Full Text]  
  • Segura, A., Godoy, P., van Dillewijn, P., Hurtado, A., Arroyo, N., Santacruz, S., Ramos, J.-L. (2005). Proteomic Analysis Reveals the Participation of Energy- and Stress-Related Proteins in the Response of Pseudomonas putida DOT-T1E to Toluene. J. Bacteriol. 187: 5937-5945 [Abstract] [Full Text]  
  • Hartig, C., Loffhagen, N., Harms, H. (2005). Formation of trans Fatty Acids Is Not Involved in Growth-Linked Membrane Adaptation of Pseudomonas putida. Appl. Environ. Microbiol. 71: 1915-1922 [Abstract] [Full Text]  
  • Rosberg-Cody, E., Ross, R. P., Hussey, S., Ryan, C. A., Murphy, B. P., Fitzgerald, G. F., Devery, R., Stanton, C. (2004). Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria. Appl. Environ. Microbiol. 70: 4635-4641 [Abstract] [Full Text]  
  • Rojas, A., Duque, E., Schmid, A., Hurtado, A., Ramos, J.-L., Segura, A. (2004). Biotransformation in Double-Phase Systems: Physiological Responses of Pseudomonas putida DOT-T1E to a Double Phase Made of Aliphatic Alcohols and Biosynthesis of Substituted Catechols. Appl. Environ. Microbiol. 70: 3637-3643 [Abstract] [Full Text]  
  • Segura, A., Hurtado, A., Duque, E., Ramos, J. L. (2004). Transcriptional Phase Variation at the flhB Gene of Pseudomonas putida DOT-T1E Is Involved in Response to Environmental Changes and Suggests the Participation of the Flagellar Export System in Solvent Tolerance. J. Bacteriol. 186: 1905-1909 [Abstract] [Full Text]  
  • Van Hamme, J. D., Singh, A., Ward, O. P. (2003). Recent Advances in Petroleum Microbiology. Microbiol. Mol. Biol. Rev. 67: 503-549 [Abstract] [Full Text]  
  • van Selm, S., van Cann, L. M., Kolkman, M. A. B., van der Zeijst, B. A. M., van Putten, J. P. M. (2003). Genetic Basis for the Structural Difference between Streptococcus pneumoniae Serotype 15B and 15C Capsular Polysaccharides. Infect. Immun. 71: 6192-6198 [Abstract] [Full Text]  
  • von Wallbrunn, A., Richnow, H. H., Neumann, G., Meinhardt, F., Heipieper, H. J. (2003). Mechanism of cis-trans Isomerization of Unsaturated Fatty Acids in Pseudomonas putida. J. Bacteriol. 185: 1730-1733 [Abstract] [Full Text]  
  • Loughlin, M. F., Jones, M. V., Lambert, P. A. (2002). Pseudomonas aeruginosa cells adapted to benzalkonium chloride show resistance to other membrane-active agents but not to clinically relevant antibiotics. J Antimicrob Chemother 49: 631-639 [Abstract] [Full Text]  
  • Godoy, P., Ramos-Gonzalez, M. I., Ramos, J. L. (2001). Involvement of the TonB System in Tolerance to Solvents and Drugs in Pseudomonas putida DOT-T1E. J. Bacteriol. 183: 5285-5292 [Abstract] [Full Text]  
  • Ramos-Gonzalez, M.-I., Godoy, P., Alaminos, M., Ben-Bassat, A., Ramos, J.-L. (2001). Physiological Characterization of Pseudomonas putida DOT-T1E Tolerance to p-Hydroxybenzoate. Appl. Environ. Microbiol. 67: 4338-4341 [Abstract] [Full Text]  
  • Segura, A., Duque, E., Hurtado, A., Ramos, J. L. (2001). Mutations in Genes Involved in the Flagellar Export Apparatus of the Solvent-Tolerant Pseudomonas putida DOT-T1E Strain Impair Motility and Lead to Hypersensitivity to Toluene Shocks. J. Bacteriol. 183: 4127-4133 [Abstract] [Full Text]  
  • Kobayashi, H., Uematsu, K., Hirayama, H., Horikoshi, K. (2000). Novel Toluene Elimination System in a Toluene-Tolerant Microorganism. J. Bacteriol. 182: 6451-6455 [Abstract] [Full Text]  
  • Halverson, L. J., Firestone, M. K. (2000). Differential Effects of Permeating and Nonpermeating Solutes on the Fatty Acid Composition of Pseudomonas putida. Appl. Environ. Microbiol. 66: 2414-2421 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»