JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petersohn, A.
Right arrow Articles by Hecker, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petersohn, A.
Right arrow Articles by Hecker, M.

Journal of Bacteriology, September 1999, p. 5718-5724, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of sigma B-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Anja Petersohn,1 Jörg Bernhardt,1 Ulf Gerth,1 Dirk Höper,1 Torsten Koburger,1 Uwe Völker,2 and Michael Hecker1,*

Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, 17487 Greifswald,1 and Laboratorium für Mikrobiologie und Max-Planck-Institut für terrestrische Mikrobiologie, Philipps-Universität Marburg, 35043 Marburg,2 Germany

Received 23 February 1999/Accepted 23 June 1999

A consensus-directed search for sigma B promoters was used to locate potential candidates for new sigma B-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wild type as well as a sigB mutant revealed 22 genes that required sigma B for induction by ethanol. Although almost 50% of the proteins encoded by the newly discovered sigma B-dependent stress genes seem to be membrane localized, biochemical functions have so far not been defined for any of the gene products. Allocation of the genes to the sigma B-dependent stress regulon may indicate a potential function in the establishment of a multiple stress resistance. AldY and YhdF show similarities to NAD(P)-dependent dehydrogenases and YdbP to thioredoxins, supporting our suggestion that sigma B-dependent proteins may be involved in the maintenance of the intracellular redox balance after stress.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Friedrich-Ludwig-Jahn-Straße 15, 17487 Greifswald, Germany. Phone: 49-3834-864200. Fax: 49-3834-864202. E-mail: hecker{at}microbio7.biologie.uni-greifswald.de.

Journal of Bacteriology, September 1999, p. 5718-5724, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 1999 by the American Society for Microbiology. All rights reserved.