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Journal of Bacteriology, September 1999, p. 5847-5851, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

CS5 Pilus Biosynthesis Genes from Enterotoxigenic Escherichia coli O115:H40

Thomas G. Duthy,1 Lothar H. Staendner,1 Paul A. Manning,1 and Michael W. Heuzenroeder2,*

Microbial Pathogenesis Unit, Department of Microbiology and Immunology, University of Adelaide, Adelaide, South Australia 5005,1 and Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000,2 Australia

Received 19 April 1999/Accepted 4 July 1999

We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in the same direction as the major subunit and are flanked by a number of insertion sequence regions have been identified. T7 polymerase-mediated overexpression of the cloned csf ORFs confirmed protein sizes based on the DNA sequences that encode them. The expression of only the csf region in E. coli K-12 resulted in the hemagglutination of human erythrocytes and the cell surface expression of CS5 pili, suggesting that the cluster contains all necessary information for CS5 pilus biogenesis and function.

* Corresponding author. Mailing address: Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia. Phone: 61 8 8222 3275. Fax: 61 8 8222 3543. E-mail: heuzenroeder{at}imvs.sa.gov.au.

Journal of Bacteriology, September 1999, p. 5847-5851, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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