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Journal of Bacteriology, September 1999, p. 5880-5884, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Archaeal Aminoacyl-tRNA Synthetase Missing from Genomic Analysis

Christian S. Hamann,1,dagger Kevin R. Sowers,2 Richard S. A. Lipman,1 and Ya-Ming Hou1,*

Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107,1 and Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022

Received 11 March 1999/Accepted 16 July 1999

The complete genomic sequencing of Methanococcus jannaschii cannot identify the gene for the cysteine-specific member of aminoacyl-tRNA synthetases. However, we show here that enzyme activity is present in the cell lysate of M. jannaschii. The demonstration of this activity suggests a direct pathway for the synthesis of cysteinyl-tRNACys during protein synthesis.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107. Phone: (215) 503-4480. Fax: (215) 923-9162. E-mail: hou1{at}jeflin.tju.edu.

dagger Present address: Merck Research Laboratories, West Point, PA 19486.

Journal of Bacteriology, September 1999, p. 5880-5884, Vol. 181, No. 18
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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