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Journal of Bacteriology, October 1999, p. 6192-6196, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Interstrain Variation of the Polysaccharide B
Biosynthesis Locus of Bacteroides fragilis:
Characterization of the Region from Strain 638R
Laurie E.
Comstock,1,*
Michael J.
Coyne,1
Arthur O.
Tzianabos,1 and
Dennis
L.
Kasper1,2
Channing Laboratory, Department of Medicine,
Brigham and Women's Hospital,1 and
Department of Microbiology and Molecular
Genetics,2 Harvard Medical School, Boston,
Massachusetts 02115
Received 10 May 1999/Accepted 15 July 1999
The sequence and analysis of the capsular polysaccharide
biosynthesis locus, PS B2, of Bacteroides fragilis 638R are
described, and the sequence is compared with that of the PS B1
biosynthesis locus of B. fragilis NCTC 9343. Two genes of
the region, wcgD and wcgC, are shown by
complementation to encode a UDP-N-acetylglucosamine 2-epimerase and a UDP-N-acetylmannosamine dehydrogenase, respectively.
*
Corresponding author. Mailing address: Channing
Laboratory, 181 Longwood Ave., Boston, MA 02115. Phone: (617) 525-2679. Fax: (617) 731-1541. E-mail:
lcomstock{at}channing.harvard.edu.
Journal of Bacteriology, October 1999, p. 6192-6196, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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