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Journal of Bacteriology, October 1999, p. 6210-6213, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functional Expression of hMYH, a Human Homolog of the Escherichia coli MutY Protein

Malgorzata M. Slupska, Wendy M. Luther, Ju-Huei Chiang, Hanjing Yang, and Jeffrey H. Miller*

Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California 90095

Received 1 June 1999/Accepted 25 July 1999

We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885-3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A · 8-hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A · G mismatch in vitro.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, CA 90095. Phone: (310) 825-3578. Fax: (310) 206-5231. E-mail: jhmiller{at}mbi.ucla.edu.

Journal of Bacteriology, October 1999, p. 6210-6213, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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