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Journal of Bacteriology, January 1999, p. 593-599, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Motility of Helicobacter pylori Is Coordinately Regulated by the Transcriptional Activator FlgR, an NtrC Homolog

Gunther Spohn and Vincenzo Scarlato*

Department of Molecular Biology, Chiron SpA, 53100 Siena, Italy

Received 10 August 1998/Accepted 22 September 1998

sigma 54 is the subunit of bacterial RNA polymerase that transcribes from promoters with enhancer elements bound by enhancer-binding proteins. By computer searches of Helicobacter pylori genomic sequences, chromosomal gene disruption, and RNA analyses, we have identified sigma 54-recognized promoters that regulate transcription of flagellar basal body and hook genes, as well as the enhancer-binding protein FlgR (flagellum regulator), a transactivating protein of the NtrC family. We demonstrate that FlgR is required for bacterial motility and transcription of five promoters for seven basal body and hook genes. In addition, FlgR acts as a repressor of transcription of the sigma 28-regulated flaA flagellin gene promoter, while changes in DNA topology repress transcription of the sigma 54-regulated flaB flagellin gene promoter. Our data indicate that regulation of flagellar gene expression in H. pylori shows similarities with that in enterobacteriaceae and Caulobacter.

* Corresponding author. Mailing address: Department of Molecular Biology, Chiron SpA, IRIS Research Center, Via Fiorentina 1, 53100 Siena, Italy. Phone: (39) 0577-243239. Fax: (39) 0577-243564. E-mail: scarlato{at}iris02.biocine.it.

Journal of Bacteriology, January 1999, p. 593-599, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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