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Journal of Bacteriology, January 1999, p. 666-669, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans: Radiation Sensitivities of Catalase (katA) and Superoxide Dismutase (sodA) Mutants

Lye Meng Markillie, Susan M. Varnum, Preston Hradecky, and Kwong-Kwok Wong*

Pacific Northwest National Laboratory, Molecular Biosciences, Richland, Washington 99352

Received 21 August 1998/Accepted 27 October 1998

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

* Corresponding author. Mailing address: Molecular Biosciences, P7-56, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352. Phone: (509) 376-5097. Fax: (509) 376-6767. E-mail: kk.wong{at}pnl.gov.

Journal of Bacteriology, January 1999, p. 666-669, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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