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Journal of Bacteriology, January 1999, p. 670-674, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli

Karinne De Pina,1 Valerie Desjardin,1 Marie-Andree Mandrand-Berthelot,1 Gerard Giordano,2 and Long-Fei Wu2,*

Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, CNRS UMR5577, Institut National des Sciences Appliquées, 69621 Villeurbanne Cedex,1 and Laboratoire de Chimie Bactérienne, CNRS UPR9043, Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20,2 France

Received 21 September 1998/Accepted 11 November 1998

Expression of the nickel-specific transport system encoded by the Escherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikR was partially controlled by Fnr through transcription from the nikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

* Corresponding author. Mailing address: Laboratoire de Chimie Bactérienne, UPR9043, Institut de Biologie Structurale et Microbiologie, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91 16 44 31. Fax: (33) 4 91 71 89 14. E-mail: wu{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, January 1999, p. 670-674, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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