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Journal of Bacteriology, October 1999, p. 6387-6395, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Ferric Enterochelin Transport in Yersinia
enterocolitica: Molecular and Evolutionary Aspects
S.
Schubert,
D.
Fischer, and
J.
Heesemann*
Max von Pettenkofer-Institut, 80336 Munich,
Germany
Received 21 June 1999/Accepted 10 August 1999
Yersinia enterocolitica is well equipped for
siderophore piracy, encompassing the utilization of siderophores such
as ferrioxamine, ferrichrome, and ferrienterochelin. In this study, we
report on the molecular and functional characterization of the
Yersinia fep-fes gene cluster orthologous to the
Escherichia coli ferrienterochelin transport genes
(fepA, fepDGC, and fepB) and the
esterase gene fes. In vitro transcription-translation
analysis identified polypeptides of 30 and 35 kDa encoded by
fepC and fes, respectively. A frameshift mutation within the fepA gene led to expression of a
truncated polypeptide of 40 kDa. The fepD,
fepG, and fes genes of Y. enterocolitica were shown to complement corresponding E. coli mutants. Insertional mutagenesis of fepD or
fes genes abrogates enterochelin-supported growth of
Y. enterocolitica on iron-chelated media. In contrast to
E. coli, the fep-fes gene cluster in
Y. enterocolitica consists solely of genes required for
uptake and utilization of enterochelin (fep) and not of
enterochelin synthesis genes such as entF. By Southern
hybridization, fepDGC and fes sequences could
be detected in Y. enterocolitica biotypes IB, IA, and II
but not in biotype IV strains, Yersinia pestis, and
Yersinia pseudotuberculosis strains. According to sequence
alignment data and the coherent structure of the Yersinia
fep-fes gene cluster, we suggest early genetic divergence of
ferrienterochelin uptake determinants among species of the family
Enterobacteriaceae.
*
Corresponding author. Mailing address: Max von
Pettenkofer-Institut, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone:
49-89-5160-5200. Fax: 49-89-5160-5223. E-mail:
heesemann{at}m3401.mpk.med.uni-muenchen.de.
Journal of Bacteriology, October 1999, p. 6387-6395, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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