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Journal of Bacteriology, October 1999, p. 6441-6448, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification and Characterization of Major Lipid Particle Proteins of the Yeast Saccharomyces cerevisiae

Karin Athenstaedt, Dagmar Zweytick, Anita Jandrositz, Sepp Dieter Kohlwein, and Günther Daum*

Institut für Biochemie und Lebensmittelchemie, Technische Universität and SFB Biomembrane Research Center, Petersgasse 12/2, A-8010 Graz, Austria

Received 11 May 1999/Accepted 11 August 1999

Lipid particles of the yeast Saccharomyces cerevisiae were isolated at high purity, and their proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Major lipid particle proteins were identified by mass spectrometric analysis, and the corresponding open reading frames (ORFs) were deduced. In silicio analysis revealed that all lipid particle proteins contain several hydrophobic domains but none or only few (hypothetical) transmembrane spanning regions. All lipid particle proteins identified by function so far, such as Erg1p, Erg6p, and Erg7p (ergosterol biosynthesis) and Faa1p, Faa4p, and Fat1p (fatty acid metabolism), are involved in lipid metabolism. Based on sequence homology, another group of three lipid particle proteins may be involved in lipid degradation. To examine whether lipid particle proteins of unknown function are also involved in lipid synthesis, mutants with deletions of the respective ORFs were constructed and subjected to systematic lipid analysis. Deletion of YDL193w resulted in a lethal phenotype which could not be suppressed by supplementation with ergosterol or fatty acids. Other deletion mutants were viable under standard conditions. Strains with YBR177c, YMR313c, and YKL140w deleted exhibited phospholipid and/or neutral lipid patterns that were different from the wild-type strain and thus may be further candidate ORFs involved in yeast lipid metabolism.

* Corresponding author. Mailing address: Institut für Biochemie und Lebensmittelchemie, Technische Universität, Petersgasse 12/2, A-8010 Graz, Austria. Phone: 43-316-873-6462. Fax: 43-316-873-6952. E-mail: f548daum{at}mbox.tu-graz.ac.at.

Journal of Bacteriology, October 1999, p. 6441-6448, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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