Previous Article | Next Article 
Journal of Bacteriology, October 1999, p. 6497-6508, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Divergent Regulation of the Evolutionarily Closely Related
Promoters of the Saccharomyces cerevisiae STA2 and
MUC1 Genes
Marco
Gagiano,
Dewald
Van Dyk,
Florian F.
Bauer,
Marius
G.
Lambrechts, and
Isak S.
Pretorius*
Institute for Wine Biotechnology and
Department of Microbiology, University of Stellenbosch,
Stellenbosch ZA-7600, South Africa
Received 26 April 1999/Accepted 3 August 1999
The 5' upstream regions of the Saccharomyces cerevisiae
glucoamylase-encoding genes STA1 to -3 and of
the MUC1 (or FLO11) gene, which is critical for
pseudohyphal development, invasive growth, and flocculation, are almost
identical, and the genes are coregulated to a large extent. Besides
representing the largest yeast promoters identified to date, these
regions are of particular interest from both a functional and an
evolutionary point of view. Transcription of the genes indeed seems to
be dependent on numerous transcription factors which integrate the
information of a complex network of signaling pathways, while the very
limited sequence differences between them should allow the study of
promoter evolution on a molecular level. To investigate the
transcriptional regulation, we compared the transcription levels
conferred by the STA2 and MUC1 promoters under
various growth conditions. Our data show that transcription of both
genes responded similarly to most environmental signals but also
indicated significant divergence in some aspects. We identified
distinct areas within the promoters that show specific responses to the
activating effect of Flo8p, Msn1p (or Mss10p, Fup1p, or Phd2p), and
Mss11p as well as to carbon catabolite repression. We also identified
the STA10 repressive effect as the absence of Flo8p, a
transcriptional activator of flocculation genes in S. cerevisiae.
*
Corresponding author. Mailing address: Institute for
Wine Biotechnology, University of Stellenbosch, Stellenbosch
ZA-7600, South Africa. Phone: 27-21-8084730. Fax: 27-21-8083771. E-mail: isp{at}maties.sun.ac.za.
Journal of Bacteriology, October 1999, p. 6497-6508, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Mao, X., Nie, X., Cao, F., Chen, J.
(2009). Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae. Acta Biochim Biophys Sin
41: 594-602
[Abstract]
[Full Text]
-
Barrales, R. R., Jimenez, J., Ibeas, J. I.
(2008). Identification of Novel Activation Mechanisms for FLO11 Regulation in Saccharomyces cerevisiae. Genetics
178: 145-156
[Abstract]
[Full Text]
-
van Dyk, D., Pretorius, I. S., Bauer, F. F.
(2005). Mss11p Is a Central Element of the Regulatory Network That Controls FLO11 Expression and Invasive Growth in Saccharomyces cerevisiae. Genetics
169: 91-106
[Abstract]
[Full Text]
-
Kim, T. S., Kim, H. Y., Yoon, J. H., Kang, H. S.
(2004). Recruitment of the Swi/Snf Complex by Ste12-Tec1 Promotes Flo8-Mss11-Mediated Activation of STA1 Expression. Mol. Cell. Biol.
24: 9542-9556
[Abstract]
[Full Text]
-
Kim, T. S., Lee, S. B., Kang, H. S.
(2004). Glucose Repression of STA1 Expression Is Mediated by the Nrg1 and Sfl1 Repressors and the Srb8-11 Complex. Mol. Cell. Biol.
24: 7695-7706
[Abstract]
[Full Text]
-
Berkey, C. D., Vyas, V. K., Carlson, M.
(2004). Nrg1 and Nrg2 Transcriptional Repressors Are Differently Regulated in Response to Carbon Source. Eukaryot Cell
3: 311-317
[Abstract]
[Full Text]
-
van Dyk, D., Hansson, G., Pretorius, I. S., Bauer, F. F.
(2003). Cellular Differentiation in Response to Nutrient Availability: The Repressor of Meiosis, Rme1p, Positively Regulates Invasive Growth in Saccharomyces cerevisiae. Genetics
165: 1045-1058
[Abstract]
[Full Text]
-
Goehring, A. S., Mitchell, D. A., Tong, A. H. Y., Keniry, M. E., Boone, C., Sprague, G. F. Jr.
(2003). Synthetic Lethal Analysis Implicates Ste20p, a p21-activated Protein Kinase, in Polarisome Activation. Mol. Biol. Cell
14: 1501-1516
[Abstract]
[Full Text]
-
Cullen, P. J., Sprague, G. F. Jr.
(2002). The Glc7p-Interacting Protein Bud14p Attenuates Polarized Growth, Pheromone Response, and Filamentous Growth in Saccharomyces cerevisiae. Eukaryot Cell
1: 884-894
[Abstract]
[Full Text]
-
Kuchin, S., Vyas, V. K., Carlson, M.
(2002). Snf1 Protein Kinase and the Repressors Nrg1 and Nrg2 Regulate FLO11, Haploid Invasive Growth, and Diploid Pseudohyphal Differentiation. Mol. Cell. Biol.
22: 3994-4000
[Abstract]
[Full Text]
-
Hohmann, S.
(2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev.
66: 300-372
[Abstract]
[Full Text]