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Journal of Bacteriology, October 1999, p. 6564-6568, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Modality of Enterobacterial Common Antigen Polysaccharide Chain Lengths Is Regulated by o349 of the wec Gene Cluster of Escherichia coli K-12

Kathleen Barr,1 John Klena,2 and Paul D. Rick1,*

Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799,1 and Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand2

Received 11 June 1999/Accepted 13 August 1999

The assembly of the phosphoglyceride-linked form of enterobacterial common antigen (ECAPG) occurs by a mechanism that involves modulation of polysaccharide chain length. However, the genetic determinant of this modulation has not been identified. Site-directed mutagenesis of o349 of the Escherichia coli K-12 wec gene cluster revealed that this locus encodes a Wzz protein that specifically modulates the chain length of ECAPG polysaccharides, and we have designated this locus wzzECA. The WzzECA-mediated modulation of ECAPG polysaccharide chains is the first demonstrated example of Wzz regulation involving a polysaccharide that is not linked to the core-lipid A structure of lipopolysaccharide.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814-4799. Phone: (301) 295-3418. Fax: (301) 295-1545. E-mail: Rickp{at}usuhs.mil.

Journal of Bacteriology, October 1999, p. 6564-6568, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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