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 Previous Article

Journal of Bacteriology, October 1999, p. 6569-6572, Vol. 181, No. 20
0021-9193/99/$04.00+0

Efficiency of Transcription from Promoter Sequence Variants in Lactobacillus Is Both Strain and Context Dependent

Andrea McCrackendagger and Peter Timms*

Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Queensland, Australia

Received 22 March 1999/Accepted 14 July 1999

The introduction of consensus -35 (TTGACA) and -10 (TATAAT) hexamers and a TG motif into the Lactobacillus acidophilus ATCC 4356 wild-type slpA promoter resulted in significant improvements (4.3-, 4.1-, and 10.7-fold, respectively) in transcriptional activity in Lactobacillus fermentum BR11. In contrast, the same changes resulted in decreased transcription in Lactobacillus rhamnosus GG. The TG motif was shown to be important in the context of weak -35 and -10 hexamers (L. fermentum BR11) or a consensus -10 hexamer (L. rhamnosus GG). Thus, both strain- and context-dependent effects are critical factors influencing transcription in Lactobacillus.

* Corresponding author. Mailing address: Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, QLD 4001, Australia. Phone: 61 7 3864 2120. Fax: 61 7 3864 1534. E-mail: p.timms{at}qut.edu.au.

dagger Present address: Department of Microbiology and Molecular Genetics, University of Texas---Houston Medical School, Houston, TX 77030.

Journal of Bacteriology, October 1999, p. 6569-6572, Vol. 181, No. 20
0021-9193/99/$04.00+0


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