This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muro-Pastor, A. M.
Right arrow Articles by Herrero, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muro-Pastor, A. M.
Right arrow Articles by Herrero, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1999, p. 6664-6669, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development

Alicia M. Muro-Pastor, Ana Valladares, Enrique Flores, and Antonia Herrero*

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, E-41092 Seville, Spain

Received 14 June 1999/Accepted 18 August 1999

The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the hetR and hetC genes. Expression of hetC, tested by RNA- DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide -571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of hetR expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for hetR induction.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio s/n, E-41092 Seville, Spain. Phone: 34-95-4489522. Fax: 34-95-4460065. E-mail: herrero{at}cica.es.

Journal of Bacteriology, November 1999, p. 6664-6669, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muro-Pastor, A. M., Flores, E., Herrero, A. (2009). NtcA-Regulated Heterocyst Differentiation Genes hetC and devB from Anabaena sp. Strain PCC 7120 Exhibit a Similar Tandem Promoter Arrangement. J. Bacteriol. 191: 5765-5774 [Abstract] [Full Text]  
  • Olmedo-Verd, E., Valladares, A., Flores, E., Herrero, A., Muro-Pastor, A. M. (2008). Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 190: 7584-7590 [Abstract] [Full Text]  
  • Valladares, A., Flores, E., Herrero, A. (2008). Transcription Activation by NtcA and 2-Oxoglutarate of Three Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 190: 6126-6133 [Abstract] [Full Text]  
  • Weyman, P. D., Pratte, B., Thiel, T. (2008). Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen. Appl. Environ. Microbiol. 74: 2103-2110 [Abstract] [Full Text]  
  • Flores, E., Pernil, R., Muro-Pastor, A. M., Mariscal, V., Maldener, I., Lechno-Yossef, S., Fan, Q., Wolk, C. P., Herrero, A. (2007). Septum-Localized Protein Required for Filament Integrity and Diazotrophy in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 3884-3890 [Abstract] [Full Text]  
  • Lopez-Gomollon, S., Hernandez, J. A., Wolk, C. P., Peleato, M. L., Fillat, M. F. (2007). Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120. Microbiology 153: 42-50 [Abstract] [Full Text]  
  • Olmedo-Verd, E., Muro-Pastor, A. M., Flores, E., Herrero, A. (2006). Localized Induction of the ntcA Regulatory Gene in Developing Heterocysts of Anabaena sp. Strain PCC 7120.. J. Bacteriol. 188: 6694-6699 [Abstract] [Full Text]  
  • Cheng, Y., Li, J.-H., Shi, L., Wang, L., Latifi, A., Zhang, C.-C. (2006). A Pair of Iron-Responsive Genes Encoding Protein Kinases with a Ser/Thr Kinase Domain and a His Kinase Domain Are Regulated by NtcA in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 4822-4829 [Abstract] [Full Text]  
  • Wang, Y., Xu, X. (2005). Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120. J. Bacteriol. 187: 8489-8493 [Abstract] [Full Text]  
  • Leitao, E., Oxelfelt, F., Oliveira, P., Moradas-Ferreira, P., Tamagnini, P. (2005). Analysis of the hupSL Operon of the Nonheterocystous Cyanobacterium Lyngbya majuscula CCAP 1446/4: Regulation of Transcription and Expression under a Light-Dark Regimen. Appl. Environ. Microbiol. 71: 4567-4576 [Abstract] [Full Text]  
  • Olmedo-Verd, E., Flores, E., Herrero, A., Muro-Pastor, A. M. (2005). HetR-Dependent and -Independent Expression of Heterocyst-Related Genes in an Anabaena Strain Overproducing the NtcA Transcription Factor. J. Bacteriol. 187: 1985-1991 [Abstract] [Full Text]  
  • Valladares, A., Muro-Pastor, A. M., Herrero, A., Flores, E. (2004). The NtcA-Dependent P1 Promoter Is Utilized for glnA Expression in N2-Fixing Heterocysts of Anabaena sp. Strain PCC 7120. J. Bacteriol. 186: 7337-7343 [Abstract] [Full Text]  
  • Oliveira, P., Leitao, E., Tamagnini, P., Moradas-Ferreira, P., Oxelfelt, F. (2004). Characterization and transcriptional analysis of hupSLW in Gloeothece sp. ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium. Microbiology 150: 3647-3655 [Abstract] [Full Text]  
  • Picossi, S., Valladares, A., Flores, E., Herrero, A. (2004). Nitrogen-regulated Genes for the Metabolism of Cyanophycin, a Bacterial Nitrogen Reserve Polymer: EXPRESSION AND MUTATIONAL ANALYSIS OF TWO CYANOPHYCIN SYNTHETASE AND CYANOPHYCINASE GENE CLUSTERS IN THE HETEROCYST-FORMING CYANOBACTERIUM ANABAENA SP. PCC 7120. J. Biol. Chem. 279: 11582-11592 [Abstract] [Full Text]  
  • Liu, D., Golden, J. W. (2002). hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 6873-6881 [Abstract] [Full Text]  
  • Koksharova, O. A., Wolk, C. P. (2002). Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 3931-3940 [Abstract] [Full Text]  
  • Meeks, J. C., Elhai, J. (2002). Regulation of Cellular Differentiation in Filamentous Cyanobacteria in Free-Living and Plant-Associated Symbiotic Growth States. Microbiol. Mol. Biol. Rev. 66: 94-121 [Abstract] [Full Text]  
  • Schmetterer, G., Valladares, A., Pils, D., Steinbach, S., Pacher, M., Muro-Pastor, A. M., Flores, E., Herrero, A. (2001). The coxBAC Operon Encodes a Cytochrome c Oxidase Required for Heterotrophic Growth in the Cyanobacterium Anabaena variabilis Strain ATCC 29413. J. Bacteriol. 183: 6429-6434 [Abstract] [Full Text]  
  • Fiedler, G., Muro-Pastor, A. M., Flores, E., Maldener, I. (2001). NtcA-Dependent Expression of the devBCA Operon, Encoding a Heterocyst-Specific ATP-Binding Cassette Transporter in Anabaena spp.. J. Bacteriol. 183: 3795-3799 [Abstract] [Full Text]  
  • Wong, F. C. Y., Meeks, J. C. (2001). The hetF Gene Product Is Essential to Heterocyst Differentiation and Affects HetR Function in the Cyanobacterium Nostoc punctiforme. J. Bacteriol. 183: 2654-2661 [Abstract] [Full Text]  
  • Muro-Pastor, A. M., Herrero, A., Flores, E. (2001). Nitrogen-Regulated Group 2 Sigma Factor from Synechocystis sp. Strain PCC 6803 Involved in Survival under Nitrogen Stress. J. Bacteriol. 183: 1090-1095 [Abstract] [Full Text]  
  • Herrero, A., Muro-Pastor, A. M., Flores, E. (2001). Nitrogen Control in Cyanobacteria. J. Bacteriol. 183: 411-425 [Full Text]  
  • Thiel, T., Pratte, B. (2001). Effect on Heterocyst Differentiation of Nitrogen Fixation in Vegetative Cells of the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 183: 280-286 [Abstract] [Full Text]  
  • Xu, X., Wolk, C. P. (2001). Role for hetC in the Transition to a Nondividing State during Heterocyst Differentiation in Anabaena sp.. J. Bacteriol. 183: 393-396 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»