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Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evidence of Horizontal Transfer of the EcoO109I Restriction-Modification Gene to Escherichia coli Chromosomal DNA

Keiko Kita,1,* Junko Tsuda,1 Toshinobu Kato,1 Kenji Okamoto,1 Hideshi Yanase,1 and Masashi Tanaka2

Department of Biotechnology, Tottori University, 4-101 Koyama, Tottori 680-8552,1 and Gifu International Institute of Biotechnology, Yagi Memorial Park, Mitake, Gifu 505-0116,2 Japan

Received 17 June 1999/Accepted 13 August 1999

A DNA fragment carrying the genes coding for EcoO109I endonuclease and EcoO109I methylase, which recognize the nucleotide sequence 5'-(A/G)GGNCC(C/T)-3', was cloned from the chromosomal DNA of Escherichia coli H709c. The EcoO109I restriction-modification (R-M) system was found to be inserted between the int and psu genes from satellite bacteriophage P4, which were lysogenized in the chromosome at the P4 phage attachment site of the corresponding leuX gene observed in E. coli K-12 chromosomal DNA. The sid gene of the prophage was inactivated by insertion of one copy of IS21. These findings may shed light on the horizontal transfer and stable maintenance of the R-M system.

* Corresponding author. Mailing address: Department of Biotechnology, Tottori University, 4-101 Koyama, Tottori 680-8552, Japan. Phone: 81-857-31-5277. Fax: 81-857-31-0881. E-mail: kita{at}bio.tottori-u.ac.jp.

Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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