Previous Article | Next Article ![]()
Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
Department of Biotechnology,
Received 17 June 1999/Accepted 13 August 1999
A DNA fragment carrying the genes coding for EcoO109I
endonuclease and EcoO109I methylase, which recognize the
nucleotide sequence 5'-(A/G)GGNCC(C/T)-3', was cloned from the
chromosomal DNA of Escherichia coli H709c. The
EcoO109I restriction-modification (R-M) system was found to
be inserted between the int and psu genes from
satellite bacteriophage P4, which were lysogenized in the chromosome at
the P4 phage attachment site of the corresponding leuX gene
observed in E. coli K-12 chromosomal DNA. The
sid gene of the prophage was inactivated by insertion of
one copy of IS21. These findings may shed light on the
horizontal transfer and stable maintenance of the R-M system.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Evidence of Horizontal Transfer of the
EcoO109I Restriction-Modification Gene to Escherichia
coli Chromosomal DNA
*
Corresponding author. Mailing address: Department of
Biotechnology, Tottori University, 4-101 Koyama, Tottori 680-8552, Japan. Phone: 81-857-31-5277. Fax: 81-857-31-0881. E-mail:
kita{at}bio.tottori-u.ac.jp.
Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
| ALL ASM JOURNALS |