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Journal of Bacteriology, November 1999, p. 6832-6835, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The BldD Protein from Streptomyces coelicolor Is a DNA-Binding Protein

Marie A. Elliot and Brenda K. Leskiw*

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9

Received 18 May 1999/Accepted 20 August 1999

Gel mobility shift assays with His-tagged BldD isolated from Escherichia coli have illustrated that BldD is capable of specifically recognizing its own promoter region. DNase I and hydroxyl radical footprinting assays have served to delimit the BldD binding site, revealing that BldD recognizes and binds to a site just upstream from, and overlapping with, the -10 region of the promoter. How BldD binds to its promoter and the effect this binding has on the expression of BldD are discussed.

* Corresponding author. Mailing address: Department of Biological Sciences, CW405 Biological Sciences Bldg., University of Alberta, Edmonton, Alberta, Canada T6G 2E9. Phone: (780) 492-1868. Fax: (780) 492-9234. E-mail: brenda.leskiw{at}ualberta.ca.

Journal of Bacteriology, November 1999, p. 6832-6835, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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