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Journal of Bacteriology, November 1999, p. 6850-6855, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Agrobacterium tumefaciens Chaperone-Like Protein, VirE1, Interacts with VirE2 at Domains Required for Single-Stranded DNA Binding and Cooperative Interactiondagger

Christopher D. Sundberg1,Dagger and Walt Ream2,*

Program in Genetics1 and Department of Microbiology,2 Oregon State University, Corvallis, Oregon 97331

Received 17 May 1999/Accepted 13 August 1999

Agrobacterium tumefaciens transfers single-stranded DNA (ssDNA) into plants. Efficient tumorigenesis requires VirE1-dependent export of ssDNA-binding (SSB) protein VirE2. VirE1 binds VirE2 domains involved in SSB and self-association, and VirE1 may facilitate VirE2 export by preventing VirE2 aggregation and the premature binding of VirE2 to ssDNA.

* Corresponding author. Mailing address: Department of Microbiology, Oregon State University, Corvallis, OR 97331. Phone: (541) 737-1791. Fax: (541) 737-0496. E-mail: reamw{at}bcc.orst.edu.

dagger Oregon State University Agricultural Experiment Station paper 11,549.

Dagger Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305.

Journal of Bacteriology, November 1999, p. 6850-6855, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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