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Journal of Bacteriology, November 1999, p. 6969-6976, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning and Characterization of the Helicobacter pylori fliD Gene, an Essential Factor in Flagellar Structure and Motility

Jang Seong Kim, Ji Hoon Chang, Soo Il Chung, and Jung Sun Yum*

Mogam Biotechnology Research Institute, Koosung-myon, Yongin-city, Kyonggi-do 449-910, Korea

Received 12 March 1999/Accepted 7 September 1999

Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma 28-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.

* Corresponding author. Mailing address: Mogam Biotechnology Research Institute, 341 Pojung-ri, Koosung-myon, Yongin-city, Kyonggi-do 449-910, Korea. Phone: 82-331-262-3851. Fax: 82-331-262-6622. E-mail: jsyum{at}kgcc.co.kr.

Journal of Bacteriology, November 1999, p. 6969-6976, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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