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Journal of Bacteriology, November 1999, p. 7131-7135, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Inactivation of the Tricarboxylic Acid Cycle Aconitase Gene from Streptomyces viridochromogenes Tü494 Impairs Morphological and Physiological Differentiation

D. Schwartz,* S. Kaspar, G. Kienzlen, K. Muschko, and W. Wohlleben

Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, D-72076 Tübingen, Germany

Received 25 June 1999/Accepted 8 September 1999

The tricarboxylic acid (TCA) cycle aconitase gene acnA from Streptomyces viridochromogenes Tü494 was cloned and analyzed. AcnA catalyzes the isomerization of citrate to isocitrate in the TCA cycle, as indicated by the ability of acnA to complement the aconitase-deficient Escherichia coli mutant JRG3259. An acnA mutant was unable to develop aerial mycelium and to sporulate, resulting in a bald phenotype. Furthermore, the mutant did not produce the antibiotic phosphinothricin tripeptide, demonstrating that AcnA also affects physiological differentiation.

* Corresponding author. Mailing address: Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. Phone: 49 7071 29-74638. Fax: 49 7071 29-5979. E-mail: schwartz{at}molbio.biol.biologie.uni-tuebingen.de.

Journal of Bacteriology, November 1999, p. 7131-7135, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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