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Journal of Bacteriology, November 1999, p. 7136-7139, Vol. 181, No. 22
Laboratorium für
Lebensmittel-Mikrobiologie, Institut für
Lebensmittelwissenschaft, Eidgenössische Technische Hochschule,
ETH-Zentrum, CH-8092 Zürich, Switzerland
Received 23 July 1999/Accepted 8 September 1999
We cloned and sequenced a 2.7-kb fragment of chromosomal DNA from
Clostridium perfringens containing the superoxide
dismutase-encoding gene, sod. Previously, rubrerythrin from
C. perfringens had been isolated and its gene
(rbr) had been cloned (Y. Lehmann, L. Meile, and M. Teuber,
J. Bacteriol. 178:7152-7158, 1996). Northern blot experiments revealed
a length of approximately 800 bases for each transcript of
rbr and sod of C. perfringens.
Thus, rbr and sod each represent a
monocistronic operon. Their transcription start points were located by
primer extension analyses. sod transcription was shown to
depend on the growth phase, and it reached a maximum during the
transition from log phase to stationary phase. Neither sod
nor rbr transcription was influenced by oxidative stress.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Transcriptional Analysis of the Rubrerythrin and
Superoxide Dismutase Genes of Clostridium
perfringens
*
Corresponding author. Mailing address: Food
Microbiology, ETH-Zentrum, CH-8092 Zürich, Switzerland. Phone:
0041-1-632 33 62. Fax: 0041-1-632 12 66. E-mail:
meile{at}ilw.agrl.ethz.ch.
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