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Journal of Bacteriology, November 1999, p. 7140-7142, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Improved Transposon for the Halophilic Archaeon Haloarcula hispanica

Wayne G. Woods, Katrina Ngui, and Michael L. Dyall-Smith*

Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia

Received 28 June 1999/Accepted 7 September 1999

An improved transposon (ThD73) for Haloarcula hispanica is described. Based on the halobacterial insertion sequence ISH28, it showed little target sequence specificity but was biased toward a lower G+C content. Twenty randomly selected ThD73 mutants were analyzed, and the DNA flanking their insertions revealed several recognizable sequences, including two (unrelated) ISH elements.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia. Phone: 61 3 9344 5693. Fax: 61 3 9347 1540. E-mail: m.dyall-smith{at}microbiol.unimelb.edu.au.

Journal of Bacteriology, November 1999, p. 7140-7142, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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