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Journal of Bacteriology, December 1999, p. 7308-7313, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Molecular Evidence for a New Bacteriophage of
Borrelia burgdorferi
Christian H.
Eggers and
D. Scott
Samuels*
Division of Biological Sciences, The
University of Montana, Missoula, Montana 59812
Received 26 July 1999/Accepted 23 September 1999
We have recovered a DNase-protected, chloroform-resistant molecule
of DNA from the cell-free supernatant of a Borrelia
burgdorferi culture. The DNA is a 32-kb double-stranded linear
molecule that is derived from the 32-kb circular plasmids (cp32s) of
the B. burgdorferi genome. Electron microscopy of samples
from which the 32-kb DNA molecule was purified revealed bacteriophage
particles. The bacteriophage has a polyhedral head with a diameter of
55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of some
B. burgdorferi strains and is inducible to higher levels with 10 µg of 1-methyl-3-nitroso-nitroguanidine (MNNG)
ml
1. In addition, the prophage can be induced with MNNG
from some Borrelia isolates that do not naturally produce
phage. We have isolated and partially characterized the phage
associated with B. burgdorferi CA-11.2A. To our knowledge,
this is the first molecular characterization of a bacteriophage of
B. burgdorferi.
*
Corresponding author. Mailing address: Division of
Biological Sciences, The University of Montana, 32 Campus Dr. #4824,
Missoula, MT 59812-4824. Phone: (406) 243-6145. Fax: (406) 243-4304. E-mail: samuels{at}selway.umt.edu.
Journal of Bacteriology, December 1999, p. 7308-7313, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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