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Journal of Bacteriology, December 1999, p. 7394-7397, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of the Bradyrhizobium japonicum ftsH Gene and Its Product

Franz Narberhaus,* Carmen Urech, and Hauke Hennecke

Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland

Received 19 July 1999/Accepted 14 September 1999

The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma 32.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Eidgenössische Technische Hochschule, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland. Phone: 41-1-632-2586. Fax: 41-1-632-1148. E-mail: fnarber{at}micro.biol.ethz.ch.

Journal of Bacteriology, December 1999, p. 7394-7397, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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