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Journal of Bacteriology, December 1999, p. 7398-7400, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Chromate Efflux by Means of the ChrA Chromate Resistance Protein from Pseudomonas aeruginosa

Angel H. Alvarez,1 Rafael Moreno-Sánchez,2 and Carlos Cervantes1,*

Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, 58030 Morelia, Mich.,1 and Departamento de Bioquímica, Instituto Nacional de Cardiología, 14080 México, D.F.,2 México

Received 28 June 1999/Accepted 21 September 1999

Everted membrane vesicles of Pseudomonas aeruginosa PAO1 harboring plasmid pCRO616, expressing the ChrA chromate resistance protein, accumulated four times more 51CrO42- than vesicles from plasmidless cells, indicating that a chromate efflux system functions in the resistant strain. Chromate uptake showed saturation kinetics with an apparent Km of 0.12 mM chromate and a Vmax of 0.5 nmol of chromate/min per mg of protein. Uptake of chromate by vesicles was dependent on NADH oxidation and was abolished by energy inhibitors and by the chromate analog sulfate. The mechanism of resistance to chromate determined by ChrA appears to be based on the active efflux of chromate driven by the membrane potential.

* Corresponding author. Mailing address: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Edificio B-3, Ciudad Universitaria, 58030 Morelia, Mich., México. Phone/fax: 52 (43) 26-5788. E-mail: cvega{at}zeus.ccu.umich.mx.

Journal of Bacteriology, December 1999, p. 7398-7400, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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