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Journal of Bacteriology, December 1999, p. 7401-7404, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Negative Control of Flagellum Synthesis in Pseudomonas
aeruginosa Is Modulated by the Alternative Sigma Factor
AlgT (AlgU)
Edward S.
Garrett,
Demetra
Perlegas, and
Daniel J.
Wozniak*
Department of Microbiology and Immunology,
Wake Forest University School of Medicine, Winston-Salem, North
Carolina 27157
Received 7 July 1999/Accepted 23 September 1999
Many respiratory isolates of Pseudomonas aeruginosa
from cystic fibrosis patients are mucoid (alginate producing) yet lack flagella. It was hypothesized that an alginate regulator inhibits flagellar gene expression. Mutations in algB,
algR, and algT resulted in nonmucoid
derivatives, yet algT mutants expressed flagella. AlgT-dependent control of flagellum synthesis occurred through inhibition of fliC but not rpoN transcription.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1064. Phone: (336) 716-2016. Fax: (336) 716-9928. E-mail: dwozniak{at}wfubmc.edu.
Journal of Bacteriology, December 1999, p. 7401-7404, Vol. 181, No. 23
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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