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Journal of Bacteriology, December 1999, p. 7409-7413, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genome-Wide Transcriptional Analysis of Aerobic and Anaerobic Chemostat Cultures of Saccharomyces cerevisiae

J. J. M. ter Linde,1 H. Liang,2 R. W. Davis,2 H. Y. Steensma,1,3,* J. P. van Dijken,3 and J. T. Pronk3

Institute of Molecular Plant Sciences, Leiden University, 2333 AL Leiden,1 and Kluyver Laboratory of Biotechnology, Delft University of Technology, 2628 BC Delft,3 The Netherlands, and Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-53072

Received 28 June 1999/Accepted 27 September 1999

The yeast Saccharomyces cerevisiae is unique among eukaryotes in exhibiting fast growth in both the presence and the complete absence of oxygen. Genome-wide transcriptional adaptation to aerobiosis and anaerobiosis was studied in assays using DNA microarrays. This technique was combined with chemostat cultivation, which allows controlled variation of a single growth parameter under defined conditions and at a fixed specific growth rate. Of the 6,171 open reading frames investigated, 5,738 (93%) yielded detectable transcript levels under either aerobic or anaerobic conditions; 140 genes showed a >3-fold-higher transcription level under anaerobic conditions. Under aerobic conditions, transcript levels of 219 genes were >3-fold higher than under anaerobic conditions.

* Corresponding author. Mailing address: Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. Phone: 31 71 5274947. Fax: 31 71 527 4999. E-mail: steensma{at}rulbim.leidenuniv.nl.

Journal of Bacteriology, December 1999, p. 7409-7413, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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