This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cusa, E.
Right arrow Articles by Aguilar, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cusa, E.
Right arrow Articles by Aguilar, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1999, p. 7479-7484, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Analysis of a Chromosomal Region Containing Genes Required for Assimilation of Allantoin Nitrogen and Linked Glyoxylate Metabolism in Escherichia coli

Eva Cusa, Nuria Obradors, Laura Baldomà, Josefa Badía, and Juan Aguilar*

Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain

Received 8 July 1999/Accepted 29 September 1999

Growth experiments with Escherichia coli have shown that this organism is able to use allantoin as a sole nitrogen source but not as a sole carbon source. Nitrogen assimilation from this compound was possible only under anaerobic conditions, in which all the enzyme activities involved in allantoin metabolism were detected. Of the nine genes encoding proteins required for allantoin degradation, only the one encoding glyoxylate carboligase (gcl), the first enzyme of the pathway leading to glycerate, had been identified and mapped at centisome 12 on the chromosome map. Phenotypic complementation of mutations in the other two genes of the glycerate pathway, encoding tartronic semialdehyde reductase (glxR) and glycerate kinase (glxK), allowed us to clone and map them closely linked to gcl. Complete sequencing of a 15.8-kb fragment encompassing these genes defined a regulon with 12 open reading frames (ORFs). Due to the high similarity of the products of two of these ORFs with yeast allantoinase and yeast allantoate amidohydrolase, a systematic analysis of the gene cluster was undertaken to identify genes involved in allantoin utilization. A BLASTP search predicted four of the genes that we sequenced to encode allantoinase (allB), allantoate amidohydrolase (allC), ureidoglycolate hydrolase (allA), and ureidoglycolate dehydrogenase (allD). The products of these genes were overexpressed and shown to have the predicted corresponding enzyme activities. Transcriptional fusions to lacZ permitted the identification of three functional promoters corresponding to three transcriptional units for the structural genes and another promoter for the regulatory gene allR. Deletion of this regulatory gene led to constitutive expression of the regulon, indicating a negatively acting function.

* Corresponding author. Mailing address: Departamento de Bioquímica, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain. Phone: 34-93-402 4521. Fax: 34-93-402 1896. E-mail: Jaguilar{at}farmacia.far.ub.es.

Journal of Bacteriology, December 1999, p. 7479-7484, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T., Tomita, M. (2009). Metabolite Profiling Reveals YihU as a Novel Hydroxybutyrate Dehydrogenase for Alternative Succinic Semialdehyde Metabolism in Escherichia coli. J. Biol. Chem. 284: 16442-16451 [Abstract] [Full Text]  
  • Pope, S. D., Chen, L.-L., Stewart, V. (2009). Purine Utilization by Klebsiella oxytoca M5al: Genes for Ring-Oxidizing and -Opening Enzymes. J. Bacteriol. 191: 1006-1017 [Abstract] [Full Text]  
  • de la Riva, L., Badia, J., Aguilar, J., Bender, R. A., Baldoma, L. (2008). The hpx Genetic System for Hypoxanthine Assimilation as a Nitrogen Source in Klebsiella pneumoniae: Gene Organization and Transcriptional Regulation. J. Bacteriol. 190: 7892-7903 [Abstract] [Full Text]  
  • Hasegawa, A., Ogasawara, H., Kori, A., Teramoto, J., Ishihama, A. (2008). The transcription regulator AllR senses both allantoin and glyoxylate and controls a set of genes for degradation and reutilization of purines. Microbiology 154: 3366-3378 [Abstract] [Full Text]  
  • Cortes, M. A. M., Gibon, J., Chanteloup, N. K., Moulin-Schouleur, M., Gilot, P., Germon, P. (2008). Inactivation of ibeA and ibeT Results in Decreased Expression of Type 1 Fimbriae in Extraintestinal Pathogenic Escherichia coli Strain BEN2908. Infect. Immun. 76: 4129-4136 [Abstract] [Full Text]  
  • Nagorska, K., Hinc, K., Strauch, M. A., Obuchowski, M. (2008). Influence of the {sigma}B Stress Factor and yxaB, the Gene for a Putative Exopolysaccharide Synthase under {sigma}B Control, on Biofilm Formation. J. Bacteriol. 190: 3546-3556 [Abstract] [Full Text]  
  • Xie, L., Bourne, P. E. (2008). Detecting evolutionary relationships across existing fold space, using sequence order-independent profile-profile alignments. Proc. Natl. Acad. Sci. USA 105: 5441-5446 [Abstract] [Full Text]  
  • Yang, C., Rodionov, D. A., Rodionova, I. A., Li, X., Osterman, A. L. (2008). Glycerate 2-Kinase of Thermotoga maritima and Genomic Reconstruction of Related Metabolic Pathways. J. Bacteriol. 190: 1773-1782 [Abstract] [Full Text]  
  • Lorca, G. L., Ezersky, A., Lunin, V. V., Walker, J. R., Altamentova, S., Evdokimova, E., Vedadi, M., Bochkarev, A., Savchenko, A. (2007). Glyoxylate and Pyruvate Are Antagonistic Effectors of the Escherichia coli IclR Transcriptional Regulator. J. Biol. Chem. 282: 16476-16491 [Abstract] [Full Text]  
  • Kang, M.-S., Besser, T. E., Hancock, D. D., Porwollik, S., McClelland, M., Call, D. R. (2006). Identification of Specific Gene Sequences Conserved in Contemporary Epidemic Strains of Salmonella enterica. Appl. Environ. Microbiol. 72: 6938-6947 [Abstract] [Full Text]  
  • Eisenhut, M., Kahlon, S., Hasse, D., Ewald, R., Lieman-Hurwitz, J., Ogawa, T., Ruth, W., Bauwe, H., Kaplan, A., Hagemann, M. (2006). The Plant-Like C2 Glycolate Cycle and the Bacterial-Like Glycerate Pathway Cooperate in Phosphoglycolate Metabolism in Cyanobacteria. Plant Physiol. 142: 333-342 [Abstract] [Full Text]  
  • Goto, M., Muramatsu, H., Mihara, H., Kurihara, T., Esaki, N., Omi, R., Miyahara, I., Hirotsu, K. (2005). Crystal Structures of {Delta}1-Piperideine-2-carboxylate/{Delta}1-Pyrroline-2-carboxylate Reductase Belonging to a New Family of NAD(P)H-dependent Oxidoreductases: CONFORMATIONAL CHANGE, SUBSTRATE RECOGNITION, AND STEREOCHEMISTRY OF THE REACTION. J. Biol. Chem. 280: 40875-40884 [Abstract] [Full Text]  
  • Fournier, D., Trott, S., Hawari, J., Spain, J. (2005). Metabolism of the Aliphatic Nitramine 4-Nitro-2,4-Diazabutanal by Methylobacterium sp. Strain JS178. Appl. Environ. Microbiol. 71: 4199-4202 [Abstract] [Full Text]  
  • Boldt, R., Edner, C., Kolukisaoglu, U., Hagemann, M., Weckwerth, W., Wienkoop, S., Morgenthal, K., Bauwe, H. (2005). D-GLYCERATE 3-KINASE, the Last Unknown Enzyme in the Photorespiratory Cycle in Arabidopsis, Belongs to a Novel Kinase Family. Plant Cell 17: 2413-2420 [Abstract] [Full Text]  
  • Muramatsu, H., Mihara, H., Kakutani, R., Yasuda, M., Ueda, M., Kurihara, T., Esaki, N. (2005). The Putative Malate/Lactate Dehydrogenase from Pseudomonas putida Is an NADPH-dependent {Delta}1-Piperideine-2-carboxylate/{Delta}1-Pyrroline-2-carboxylate Reductase Involved in the Catabolism of D-Lysine and D-Proline. J. Biol. Chem. 280: 5329-5335 [Abstract] [Full Text]  
  • Chou, H.-C., Lee, C.-Z., Ma, L.-C., Fang, C.-T., Chang, S.-C., Wang, J.-T. (2004). Isolation of a Chromosomal Region of Klebsiella pneumoniae Associated with Allantoin Metabolism and Liver Infection. Infect. Immun. 72: 3783-3792 [Abstract] [Full Text]  
  • Mulrooney, S. B., Hausinger, R. P. (2003). Metal Ion Dependence of Recombinant Escherichia coli Allantoinase. J. Bacteriol. 185: 126-134 [Abstract] [Full Text]  
  • Beier, L., Nygaard, P., Jarmer, H., Saxild, H. H. (2002). Transcription Analysis of the Bacillus subtilis PucR Regulon and Identification of a cis-Acting Sequence Required for PucR-Regulated Expression of Genes Involved in Purine Catabolism. J. Bacteriol. 184: 3232-3241 [Abstract] [Full Text]  
  • Garaizar, J., Porwollik, S., Echeita, A., Rementeria, A., Herrera, S., Wong, R. M.-Y., Frye, J., Usera, M. A., McClelland, M. (2002). DNA Microarray-Based Typing of an Atypical Monophasic Salmonella enterica Serovar. J. Clin. Microbiol. 40: 2074-2078 [Abstract] [Full Text]  
  • Oh, M.-K., Rohlin, L., Kao, K. C., Liao, J. C. (2002). Global Expression Profiling of Acetate-grown Escherichia coli. J. Biol. Chem. 277: 13175-13183 [Abstract] [Full Text]  
  • Reitzer, L., Schneider, B. L. (2001). Metabolic Context and Possible Physiological Themes of {sigma}54-Dependent Genes in Escherichia coli. Microbiol. Mol. Biol. Rev. 65: 422-444 [Abstract] [Full Text]  
  • Schultz, A. C., Nygaard, P., Saxild, H. H. (2001). Functional Analysis of 14 Genes That Constitute the Purine Catabolic Pathway in Bacillus subtilis and Evidence for a Novel Regulon Controlled by the PucR Transcription Activator. J. Bacteriol. 183: 3293-3302 [Abstract] [Full Text]  
  • Lowe, C. A., Asghar, A. H., Shalom, G., Shaw, J. G., Thomas, M. S. (2001). The Burkholderia cepacia fur gene: co-localization with omlA and absence of regulation by iron. Microbiology 147: 1303-1314 [Abstract] [Full Text]  
  • Kim, G. J., Lee, D. E., Kim, H.-S. (2000). Functional Expression and Characterization of the Two Cyclic Amidohydrolase Enzymes, Allantoinase and a Novel Phenylhydantoinase, from Escherichia coli. J. Bacteriol. 182: 7021-7028 [Abstract] [Full Text]  
  • Xi, H., Schneider, B. L., Reitzer, L. (2000). Purine Catabolism in Escherichia coli and Function of Xanthine Dehydrogenase in Purine Salvage. J. Bacteriol. 182: 5332-5341 [Abstract] [Full Text]  
  • Monterrubio, R., Baldoma, L., Obradors, N., Aguilar, J., Badia, J. (2000). A Common Regulator for the Operons Encoding the Enzymes Involved in D-Galactarate, D-Glucarate, and D-Glycerate Utilization in Escherichia coli. J. Bacteriol. 182: 2672-2674 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»