This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Porta, A.
Right arrow Articles by Fonzi, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Porta, A.
Right arrow Articles by Fonzi, W. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1999, p. 7516-7523, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PRR1, a Homolog of Aspergillus nidulans palF, Controls pH-Dependent Gene Expression and Filamentation in Candida albicans

Amalia Porta, Ana M. Ramon, and William A. Fonzi*

Department of Microbiology and Immunology, Georgetown University, Washington, D.C. 20007-2197

Received 3 August 1999/Accepted 29 September 1999

The pH of the environment has been implicated in controlling the yeast-hypha transition and pathogenesis of Candida albicans. Several C. albicans genes, including PHR1 and PHR2, are pH dependent in their expression. To investigate the mechanism of pH-dependent expression, we have cloned and characterized PRR1 (for pH response regulator). PRR1 is homologous to palF, a component of the pH response pathway in Aspergillus nidulans. Expression of PRR1 was itself pH dependent, being maximal at acid pH but reduced severalfold at alkaline pH. In a prr1 null mutant the alkaline-induced expression of PHR1 was completely abolished. Conversely, expression of PHR2 was no longer repressed at alkaline pH. A prr1 null mutant exhibited no morphological abnormalities at either pH; however, it lost the ability to form hyphae on medium 199 and on 10% serum plates. The ability to filament on serum was not restored by forced expression of PHR1, indicating that additional PRR1-dependent genes are required for hyphal development. These developmental genes appear to be distinct from those controlled by the developmental regulator EFG1, since the EFG1-dependent gene HWP1 was expressed normally in the prr1 null mutant. We conclude that PRR1 encodes a component of the pH-dependent response pathway in C. albicans and that this pathway regulates the expression of multiple components of hyphal development.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Georgetown University, 3900 Reservoir Rd., N.W., Washington, DC 20007-2197. Phone: (202) 687-1135. Fax: (202) 687-1800. E-mail: fonziw{at}gusun.georgetown.edu.

Journal of Bacteriology, December 1999, p. 7516-7523, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sagaram, U. S., Shaw, B. D., Shim, W.-B. (2007). Fusarium verticillioides GAP1, a gene encoding a putative glycolipid-anchored surface protein, participates in conidiation and cell wall structure but not virulence. Microbiology 153: 2850-2861 [Abstract] [Full Text]  
  • Zhao, X., Oh, S.-H., Hoyer, L. L. (2007). Unequal contribution of ALS9 alleles to adhesion between Candida albicans and human vascular endothelial cells. Microbiology 153: 2342-2350 [Abstract] [Full Text]  
  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Mitchell, B. M., Wu, T. G., Jackson, B. E., Wilhelmus, K. R. (2007). Candida albicans Strain-Dependent Virulence and Rim13p-Mediated Filamentation in Experimental Keratomycosis. IOVS 48: 774-780 [Abstract] [Full Text]  
  • Baek, Y.-U., Martin, S. J., Davis, D. A. (2006). Evidence for Novel pH-Dependent Regulation of Candida albicans Rim101, a Direct Transcriptional Repressor of the Cell Wall {beta}-Glycosidase Phr2.. Eukaryot Cell 5: 1550-1559 [Abstract] [Full Text]  
  • Zhao, X., Daniels, K. J., Oh, S.-H., Green, C. B., Yeater, K. M., Soll, D. R., Hoyer, L. L. (2006). Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces.. Microbiology 152: 2287-2299 [Abstract] [Full Text]  
  • Cornet, M., Bidard, F., Schwarz, P., Da Costa, G., Blanchin-Roland, S., Dromer, F., Gaillardin, C. (2005). Deletions of Endocytic Components VPS28 and VPS32 Affect Growth at Alkaline pH and Virulence through both RIM101-Dependent and RIM101-Independent Pathways in Candida albicans. Infect. Immun. 73: 7977-7987 [Abstract] [Full Text]  
  • Cheng, S., Clancy, C. J., Checkley, M. A., Zhang, Z., Wozniak, K. L., Seshan, K. R., Jia, H. Y., Fidel, P. Jr, Cole, G., Nguyen, M. H. (2005). The Role of Candida albicans NOT5 in Virulence Depends upon Diverse Host Factors In Vivo. Infect. Immun. 73: 7190-7197 [Abstract] [Full Text]  
  • Hayashi, M., Fukuzawa, T., Sorimachi, H., Maeda, T. (2005). Constitutive Activation of the pH-Responsive Rim101 Pathway in Yeast Mutants Defective in Late Steps of the MVB/ESCRT Pathway. Mol. Cell. Biol. 25: 9478-9490 [Abstract] [Full Text]  
  • Veses, V., Casanova, M., Murgui, A., Dominguez, A., Gow, N. A. R., Martinez, J. P. (2005). ABG1, a Novel and Essential Candida albicans Gene Encoding a Vacuolar Protein Involved in Cytokinesis and Hyphal Branching. Eukaryot Cell 4: 1088-1101 [Abstract] [Full Text]  
  • Zhao, X., Oh, S.-H., Yeater, K. M., Hoyer, L. L. (2005). Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family. Microbiology 151: 1619-1630 [Abstract] [Full Text]  
  • Green, C. B., Zhao, X., Yeater, K. M., Hoyer, L. L. (2005). Construction and real-time RT-PCR validation of Candida albicans PALS-GFP reporter strains and their use in flow cytometry analysis of ALS gene expression in budding and filamenting cells. Microbiology 151: 1051-1060 [Abstract] [Full Text]  
  • Sharkey, L. L., Liao, W.-l., Ghosh, A. K., Fonzi, W. A. (2005). Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans. Microbiology 151: 1061-1071 [Abstract] [Full Text]  
  • Oh, S.-H., Cheng, G., Nuessen, J. A., Jajko, R., Yeater, K. M., Zhao, X., Pujol, C., Soll, D. R., Hoyer, L. L. (2005). Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain. Microbiology 151: 673-681 [Abstract] [Full Text]  
  • Kullas, A. L., Li, M., Davis, D. A. (2004). Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans. Eukaryot Cell 3: 1609-1618 [Abstract] [Full Text]  
  • Xu, W., Smith, F. J. Jr., Subaran, R., Mitchell, A. P. (2004). Multivesicular Body-ESCRT Components Function in pH Response Regulation in Saccharomyces cerevisiae and Candida albicans. Mol. Biol. Cell 15: 5528-5537 [Abstract] [Full Text]  
  • Nicholls, S., Straffon, M., Enjalbert, B., Nantel, A., Macaskill, S., Whiteway, M., Brown, A. J. P. (2004). Msn2- and Msn4-Like Transcription Factors Play No Obvious Roles in the Stress Responses of the Fungal Pathogen Candida albicans. Eukaryot Cell 3: 1111-1123 [Abstract] [Full Text]  
  • Galan, A., Casanova, M., Murgui, A., MacCallum, D. M., Odds, F. C., Gow, N. A. R., Martinez, J. P. (2004). The Candida albicans pH-regulated KER1 gene encodes a lysine/glutamic-acid-rich plasma-membrane protein that is involved in cell aggregation. Microbiology 150: 2641-2651 [Abstract] [Full Text]  
  • Zhao, X., Oh, S.-H., Cheng, G., Green, C. B., Nuessen, J. A., Yeater, K., Leng, R. P., Brown, A. J. P., Hoyer, L. L. (2004). ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparisons between Als3p and Als1p. Microbiology 150: 2415-2428 [Abstract] [Full Text]  
  • Li, M., Martin, S. J., Bruno, V. M., Mitchell, A. P., Davis, D. A. (2004). Candida albicans Rim13p, a Protease Required for Rim101p Processing at Acidic and Alkaline pHs. Eukaryot Cell 3: 741-751 [Abstract] [Full Text]  
  • Nobile, C. J., Bruno, V. M., Richard, M. L., Davis, D. A., Mitchell, A. P. (2003). Genetic control of chlamydospore formation in Candida albicans. Microbiology 149: 3629-3637 [Abstract] [Full Text]  
  • Cheng, S., Nguyen, M. H., Zhang, Z., Jia, H., Handfield, M., Clancy, C. J. (2003). Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus. Infect. Immun. 71: 6101-6103 [Abstract] [Full Text]  
  • Spreghini, E., Davis, D. A., Subaran, R., Kim, M., Mitchell, A. P. (2003). Roles of Candida albicans Dfg5p and Dcw1p Cell Surface Proteins in Growth and Hypha Formation. Eukaryot Cell 2: 746-755 [Abstract] [Full Text]  
  • Blankenship, J. R., Wormley, F. L., Boyce, M. K., Schell, W. A., Filler, S. G., Perfect, J. R., Heitman, J. (2003). Calcineurin Is Essential for Candida albicans Survival in Serum and Virulence. Eukaryot Cell 2: 422-430 [Abstract] [Full Text]  
  • Lamb, T. M., Mitchell, A. P. (2003). The Transcription Factor Rim101p Governs Ion Tolerance and Cell Differentiation by Direct Repression of the Regulatory Genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 677-686 [Abstract] [Full Text]  
  • Davis, D. A., Bruno, V. M., Loza, L., Filler, S. G., Mitchell, A. P. (2002). Candida albicans Mds3p, a Conserved Regulator of pH Responses and Virulence Identified Through Insertional Mutagenesis. Genetics 162: 1573-1581 [Abstract] [Full Text]  
  • Nantel, A., Dignard, D., Bachewich, C., Harcus, D., Marcil, A., Bouin, A.-P., Sensen, C. W., Hogues, H., van het Hoog, M., Gordon, P., Rigby, T., Benoit, F., Tessier, D. C., Thomas, D. Y., Whiteway, M. (2002). Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition. Mol. Biol. Cell 13: 3452-3465 [Abstract] [Full Text]  
  • Penalva, M. A., Arst, H. N. Jr. (2002). Regulation of Gene Expression by Ambient pH in Filamentous Fungi and Yeasts. Microbiol. Mol. Biol. Rev. 66: 426-446 [Abstract] [Full Text]  
  • McNemar, M. D., Fonzi, W. A. (2002). Conserved Serine/Threonine Kinase Encoded by CBK1 Regulates Expression of Several Hypha-Associated Transcripts and Genes Encoding Cell Wall Proteins in Candida albicans. J. Bacteriol. 184: 2058-2061 [Abstract] [Full Text]  
  • Gonzalez-Lopez, C. I., Szabo, R., Blanchin-Roland, S., Gaillardin, C. (2002). Genetic Control of Extracellular Protease Synthesis in the Yeast Yarrowia lipolytica. Genetics 160: 417-427 [Abstract] [Full Text]  
  • Mingot, J. M., Espeso, E. A., Díez, E., Peñalva, M. A. (2001). Ambient pH Signaling Regulates Nuclear Localization of the Aspergillus nidulans PacC Transcription Factor. Mol. Cell. Biol. 21: 1688-1699 [Abstract] [Full Text]  
  • Andaluz, E., Calderone, R., Reyes, G., Larriba, G. (2001). Phenotypic Analysis and Virulence of Candida albicans LIG4 Mutants. Infect. Immun. 69: 137-147 [Abstract] [Full Text]  
  • Davis, D., Edwards, J. E. Jr., Mitchell, A. P., Ibrahim, A. S. (2000). Candida albicans RIM101 pH Response Pathway Is Required for Host-Pathogen Interactions. Infect. Immun. 68: 5953-5959 [Abstract] [Full Text]  
  • Ernst, J. F. (2000). Transcription factors in Candida albicans - environmental control of morphogenesis. Microbiology 146: 1763-1774 [Full Text]  
  • El Barkani, A., Kurzai, O., Fonzi, W. A., Ramon, A., Porta, A., Frosch, M., Mühlschlegel, F. A. (2000). Dominant Active Alleles of RIM101 (PRR2) Bypass the pH Restriction on Filamentation of Candida albicans. Mol. Cell. Biol. 20: 4635-4647 [Abstract] [Full Text]  
  • Ramon, A. M., Porta, A., Fonzi, W. A. (1999). Effect of Environmental pH on Morphological Development of Candida albicans Is Mediated via the PacC-Related Transcription Factor Encoded by PRR2. J. Bacteriol. 181: 7524-7530 [Abstract] [Full Text]  
  • Lamb, T. M., Xu, W., Diamond, A., Mitchell, A. P. (2001). Alkaline Response Genes of Saccharomyces cerevisiae and Their Relationship to the RIM101 Pathway. J. Biol. Chem. 276: 1850-1856 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»