This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wyckoff, E. E.
Right arrow Articles by Payne, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wyckoff, E. E.
Right arrow Articles by Payne, S. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1999, p. 7588-7596, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Multifunctional ATP-Binding Cassette Transporter System from Vibrio cholerae Transports Vibriobactin and Enterobactin

Elizabeth E. Wyckoff,* Ana-Maria Valle, Stacey L. Smith, and Shelley M. Payne

Department of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712-1095

Received 14 July 1999/Accepted 5 October 1999

Vibrio cholerae uses the catechol siderophore vibriobactin for iron transport under iron-limiting conditions. We have identified genes for vibriobactin transport and mapped them within the vibriobactin biosynthetic gene cluster. Within this genetic region we have identified four genes, viuP, viuD, viuG and viuC, whose protein products have homology to the periplasmic binding protein, the two integral cytoplasmic membrane proteins, and the ATPase component, respectively, of other iron transport systems. The amino-terminal region of ViuP has homology to a lipoprotein signal sequence, and ViuP could be labeled with [3H]palmitic acid. This suggests that ViuP is a membrane lipoprotein. The ViuPDGC system transports both vibriobactin and enterobactin in Escherichia coli. In the same assay, the E. coli enterobactin transport system, FepBDGC, allowed the utilization of enterobactin but not vibriobactin. Although the entire viuPDGC system could complement mutations in fepB, fepD, fepG, or fepC, only viuC was able to independently complement the corresponding fep mutation. This indicates that these proteins usually function as a complex. V. cholerae strains carrying a mutation in viuP or in viuG were constructed by marker exchange. These mutations reduced, but did not completely eliminate, vibriobactin utilization. This suggests that V. cholerae contains genes in addition to viuPDGC that function in the transport of catechol siderophores.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, University of Texas, Austin, TX 78712-1095. Phone: (512) 471-5204. Fax: (512) 471-7088. E-mail: ewyckoff{at}mail.utexas.edu.

Journal of Bacteriology, December 1999, p. 7588-7596, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lin, A. E., Krastel, K., Hobb, R. I., Thompson, S. A., Cvitkovitch, D. G., Gaynor, E. C. (2009). Atypical Roles for Campylobacter jejuni Amino Acid ATP Binding Cassette Transporter Components PaqP and PaqQ in Bacterial Stress Tolerance and Pathogen-Host Cell Dynamics. Infect. Immun. 77: 4912-4924 [Abstract] [Full Text]  
  • Sikora, A. E., Beyhan, S., Bagdasarian, M., Yildiz, F. H., Sandkvist, M. (2009). Cell Envelope Perturbation Induces Oxidative Stress and Changes in Iron Homeostasis in Vibrio cholerae. J. Bacteriol. 191: 5398-5408 [Abstract] [Full Text]  
  • Miethke, M., Marahiel, M. A. (2007). Siderophore-Based Iron Acquisition and Pathogen Control. Microbiol. Mol. Biol. Rev. 71: 413-451 [Abstract] [Full Text]  
  • Wyckoff, E. E., Mey, A. R., Leimbach, A., Fisher, C. F., Payne, S. M. (2006). Characterization of Ferric and Ferrous Iron Transport Systems in Vibrio cholerae.. J. Bacteriol. 188: 6515-6523 [Abstract] [Full Text]  
  • Mey, A. R., Wyckoff, E. E., Kanukurthy, V., Fisher, C. R., Payne, S. M. (2005). Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence. Infect. Immun. 73: 8167-8178 [Abstract] [Full Text]  
  • Mey, A. R., Craig, S. A., Payne, S. M. (2005). Characterization of Vibrio cholerae RyhB: the RyhB Regulon and Role of ryhB in Biofilm Formation. Infect. Immun. 73: 5706-5719 [Abstract] [Full Text]  
  • Anderson, M. T., Armstrong, S. K. (2004). The BfeR Regulator Mediates Enterobactin-Inducible Expression of Bordetella Enterobactin Utilization Genes. J. Bacteriol. 186: 7302-7311 [Abstract] [Full Text]  
  • Fernandez, L., Marquez, I., Guijarro, J. A. (2004). Identification of Specific In Vivo-Induced (ivi) Genes in Yersinia ruckeri and Analysis of Ruckerbactin, a Catecholate Siderophore Iron Acquisition System. Appl. Environ. Microbiol. 70: 5199-5207 [Abstract] [Full Text]  
  • Wyckoff, E. E., Schmitt, M., Wilks, A., Payne, S. M. (2004). HutZ Is Required for Efficient Heme Utilization in Vibrio cholerae. J. Bacteriol. 186: 4142-4151 [Abstract] [Full Text]  
  • Ciche, T. A., Blackburn, M., Carney, J. R., Ensign, J. C. (2003). Photobactin: a Catechol Siderophore Produced by Photorhabdus luminescens, an Entomopathogen Mutually Associated with Heterorhabditis bacteriophora NC1 Nematodes. Appl. Environ. Microbiol. 69: 4706-4713 [Abstract] [Full Text]  
  • Raymond, K. N., Dertz, E. A., Kim, S. S. (2003). Bioinorganic Chemistry Special Feature: Enterobactin: An archetype for microbial iron transport. Proc. Natl. Acad. Sci. USA 100: 3584-3588 [Abstract] [Full Text]  
  • Mey, A. R., Payne, S. M. (2003). Analysis of Residues Determining Specificity of Vibrio cholerae TonB1 for Its Receptors. J. Bacteriol. 185: 1195-1207 [Abstract] [Full Text]  
  • Mey, A. R., Wyckoff, E. E., Oglesby, A. G., Rab, E., Taylor, R. K., Payne, S. M. (2002). Identification of the Vibrio cholerae Enterobactin Receptors VctA and IrgA: IrgA Is Not Required for Virulence. Infect. Immun. 70: 3419-3426 [Abstract] [Full Text]  
  • Crosa, J. H., Walsh, C. T. (2002). Genetics and Assembly Line Enzymology of Siderophore Biosynthesis in Bacteria. Microbiol. Mol. Biol. Rev. 66: 223-249 [Abstract] [Full Text]  
  • Panina, E. M., Mironov, A. A., Gelfand, M. S. (2001). Comparative analysis of FUR regulons in gamma-proteobacteria. Nucleic Acids Res 29: 5195-5206 [Abstract] [Full Text]  
  • Henderson, D. P., Wyckoff, E. E., Rashidi, C. E., Verlei, H., Oldham, A. L. (2001). Characterization of the Plesiomonas shigelloides Genes Encoding the Heme Iron Utilization System. J. Bacteriol. 183: 2715-2723 [Abstract] [Full Text]  
  • Wyckoff, E. E., Smith, S. L., Payne, S. M. (2001). VibD and VibH Are Required for Late Steps in Vibriobactin Biosynthesis in Vibrio cholerae. J. Bacteriol. 183: 1830-1834 [Abstract] [Full Text]  
  • Sprencel, C., Cao, Z., Qi, Z., Scott, D. C., Montague, M. A., Ivanoff, N., Xu, J., Raymond, K. M., Newton, S. M. C., Klebba, P. E. (2000). Binding of Ferric Enterobactin by the Escherichia coli Periplasmic Protein FepB. J. Bacteriol. 182: 5359-5364 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»