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Journal of Bacteriology, December 1999, p. 7643-7646, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Structural and Functional Analyses of the Secondary Cell Wall Polymer of Bacillus sphaericus CCM 2177 That Serves as an S-Layer-Specific Anchor

Nicola Ilk,1 Paul Kosma,2 Michael Puchberger,2 Eva M. Egelseer,1 Harald F. Mayer,1 Uwe B. Sleytr,1 and Margit Sára1,*

Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie1 and Institut für Chemie,2 University of Agricultural Sciences, 1180 Vienna, Austria

Received 15 July 1999/Accepted 4 October 1999

Sacculi of Bacillus sphaericus CCM 2177 contain a secondary cell wall polymer which was completely extracted with 48% hydrofluoric acid. Nuclear magnetic resonance analysis showed that the polymer is composed of repeating units, as follows: right-arrow3)-[4,6-O-(1-carboxyethylidene)]~0.5-beta -D-ManpNAc-(1right-arrow4)-beta -D-GlcpNAc-(1right-arrow. The N-terminal part of the S-layer protein carrying S-layer homologous motifs recognizes this polymer as a binding site.

* Corresponding author. Mailing address: Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Gregor-Mendelstr. 33, 1180 Vienna, Austria. Phone: 0043-1-47654/2208. Fax: 0043-1-478 91 12. E-mail: sara{at}edv1.boku.ac.at.

Journal of Bacteriology, December 1999, p. 7643-7646, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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