This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, Z.
Right arrow Articles by Forbes, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, Z.
Right arrow Articles by Forbes, K. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 1999, p. 1021-1024, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of IS1547, a New Member of the IS900 Family in the Mycobacterium tuberculosis Complex, and Its Association with IS6110

Z. Fang,1 C. Doig,2 N. Morrison,1 B. Watt,2 and K. J. Forbes1,*

Medical Microbiology, Aberdeen University, Foresterhill, Aberdeen AB25 2ZD,1 and Scottish Mycobacteria Reference Laboratory, The City Hospital, Edinburgh EH10 5SB,2 United Kingdom

Received 23 February 1998/Accepted 23 November 1998

Unlike classically defined insertion sequence (IS) elements, which are delimited by their inverted terminal repeats, some IS elements do not have inverted terminal repeats. Among this group of atypical IS elements, IS116, IS900, IS901, and IS1110 have been proposed as members of the IS900 family of elements, not only because they do not have inverted terminal repeats but also because they share other features such as homologous transposases and particular insertion sites. In this study, we report a newly identified IS sequence, IS1547, which was first identified in a clinical isolate of Mycobacterium tuberculosis. Its structure, insertion site, and putative transposase all conform with the conventions of the IS900 family, suggesting that it is a new member of this family. IS1547 was detected only in isolates of the M. tuberculosis complex, where it had highly polymorphic restriction fragment length polymorphism patterns, suggesting that it may be a useful genetic marker for identifying isolates of the M. tuberculosis complex and for distinguishing different strains of M. tuberculosis. ipl is a preferential locus for IS6110 insertion where there are eight known different insertion sites for IS6110. Surprisingly, the DNA sequence of ipl is now known to be a part of IS1547, meaning that IS1547 is a preferential site for IS6110 insertion.

* Corresponding author. Mailing address: Medical Microbiology, Aberdeen University, Foresterhill, Aberdeen AB25 2ZD, United Kingdom. Phone: 44 1224 663123, ext. 54953. Fax: 44 1224 685604. E-mail: mmb001{at}abdn.ac.uk.

Journal of Bacteriology, February 1999, p. 1021-1024, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Otsuka, Y., Parniewski, P., Zwolska, Z., Kai, M., Fujino, T., Kirikae, F., Toyota, E., Kudo, K., Kuratsuji, T., Kirikae, T. (2004). Characterization of a Trinucleotide Repeat Sequence (CGG)5 and Potential Use in Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 3538-3548 [Abstract] [Full Text]  
  • Dale, J. W., Al-Ghusein, H., Al-Hashmi, S., Butcher, P., Dickens, A. L., Drobniewski, F., Forbes, K. J., Gillespie, S. H., Lamprecht, D., McHugh, T. D., Pitman, R., Rastogi, N., Smith, A. T., Sola, C., Yesilkaya, H. (2003). Evolutionary Relationships among Strains of Mycobacterium tuberculosis with Few Copies of IS6110. J. Bacteriol. 185: 2555-2562 [Abstract] [Full Text]  
  • Kivi, M., Liu, X., Raychaudhuri, S., Altman, R. B., Small, P. M. (2002). Determining the Genomic Locations of Repetitive DNA Sequences with a Whole-Genome Microarray: IS6110 in Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 2192-2198 [Abstract] [Full Text]  
  • Doig, C., Muckersie, L., Watt, B., Forbes, K. J. (2002). Molecular Epidemiology of Mycobacterium malmoense Infections in Scotland. J. Clin. Microbiol. 40: 1103-1105 [Abstract] [Full Text]  
  • Fang, Z., Kenna, D. T., Doig, C., Smittipat, D. N., Palittapongarnpim, P., Watt, B., Forbes, K. J. (2001). Molecular Evidence for Independent Occurrence of IS6110 Insertions at the Same Sites of the Genome of Mycobacterium tuberculosis in Different Clinical Isolates. J. Bacteriol. 183: 5279-5284 [Abstract] [Full Text]  
  • Harris, N. B., Barletta, R. G. (2001). Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine. Clin. Microbiol. Rev. 14: 489-512 [Abstract] [Full Text]  
  • Legrand, E., Filliol, I., Sola, C., Rastogi, N. (2001). Use of Spoligotyping To Study the Evolution of the Direct Repeat Locus by IS6110 Transposition in Mycobacterium tuberculosis. J. Clin. Microbiol. 39: 1595-1599 [Abstract] [Full Text]  
  • Eckstein, T. M., Inamine, J. M., Lambert, M. L., Belisle, J. T. (2000). A Genetic Mechanism for Deletion of the ser2 Gene Cluster and Formation of Rough Morphological Variants of Mycobacterium avium. J. Bacteriol. 182: 6177-6182 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»