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Journal of Bacteriology, February 1999, p. 1025-1029, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of a Gene (mspA) Encoding the Major Sheath Protein of Treponema maltophilum ATCC 51939T

Klaus Heuner,1 Bong-Kyu Choi,2 Rüdiger Schade,3 Annette Moter,1 Albrecht Otto,4 and Ulf B. Göbel1,*

Institut für Mikrobiologie und Hygiene1 and Institut für Pharmakologie und Toxikologie,3 Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, and Max-Delbrück-Centrum für Molekulare Medizin, Berlin-Buch,4 Germany, and Department of Oral Biology, College of Dentistry, Yonsei University, 120-752 Seoul, Korea2

Received 17 August 1998/Accepted 21 November 1998

The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939T was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal peptide of 19 amino acids. The native MspA formed a heat-modifiable, detergent- and trypsin-stable complex which is associated with the outer membrane. Hybridization with an mspA-specific probe showed no cross-reactivity with the msp gene from Treponema denticola.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Berlin, Germany. Phone: 49-3020934715. Fax: 49-3020934703. E-mail: ulf.goebel{at}charite.de.

Journal of Bacteriology, February 1999, p. 1025-1029, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Edwards, A. M., Jenkinson, H. F., Woodward, M. J., Dymock, D. (2005). Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405. Infect. Immun. 73: 2891-2898 [Abstract] [Full Text]  
  • Lee, S.-H., Kim, K.-K., Choi, B.-K. (2005). Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum, the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections. Infect. Immun. 73: 268-276 [Abstract] [Full Text]  


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