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Journal of Bacteriology, February 1999, p. 1045-1048, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Transfer of Large DNA Inserts to Designated Loci of the Bacillus subtilis 168 Genome

Mitsuhiro Itaya*

Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo 194-8511, Japan

Received 6 August 1998/Accepted 19 November 1998

It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created.

* Mailing address: Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. Phone: 81-42-724-6254. Fax: 81-42-724-6316.

Journal of Bacteriology, February 1999, p. 1045-1048, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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