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 Previous Article

Journal of Bacteriology, February 1999, p. 1054-1058, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Two Roles for the Leucine-Responsive Regulatory Protein in Expression of the Alanine Catabolic Operon (dadAB) in Klebsiella aerogenes

Brian K. Janes and Robert A. Bender*

Department of Biology, The University of Michigan, Ann Arbor, Michigan 49109-1048

Received 11 September 1998/Accepted 16 November 1998

The lrp gene, which codes for the leucine-responsive regulatory protein (Lrp), was cloned from Klebsiella aerogenes W70. The DNA sequence was determined, and the clone was used to create a disruption of the lrp gene. The lack of functional Lrp led to an increased expression of the alanine catabolic operon (dad) in the absence of the inducer L-alanine but also to a decreased expression of the operon in the presence of L-alanine. Thus, Lrp is both a repressor and activator of dad expression. Lrp is also necessary for glutamate synthase formation but not for the formation of two other enzymes controlled by the nitrogen regulatory (Ntr) system, glutamate dehydrogenase and histidase.

* Corresponding author. Mailing address: Department of Biology, The University of Michigan, Ann Arbor, MI 49109-1048. Phone: (734) 936-2530. Fax: (734) 647-0884. E-mail: rbender{at}umich.edu.

Journal of Bacteriology, February 1999, p. 1054-1058, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Belitsky, B. R., Wray, L. V. Jr., Fisher, S. H., Bohannon, D. E., Sonenshein, A. L. (2000). Role of TnrA in Nitrogen Source-Dependent Repression of Bacillus subtilis Glutamate Synthase Gene Expression. J. Bacteriol. 182: 5939-5947 [Abstract] [Full Text]  


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