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Journal of Bacteriology, February 1999, p. 998-1004, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The SPI-3 Pathogenicity Island of Salmonella enterica

Anne-Béatrice Blanc-Potard, Felix Solomon, Jayson Kayser, and Eduardo A. Groisman*

Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110

Received 9 October 1998/Accepted 12 November 1998

Pathogenicity islands are chromosomal clusters of pathogen-specific virulence genes often found at tRNA loci. We have determined the molecular genetic structure of SPI-3, a 17-kb pathogenicity island located at the selC tRNA locus of Salmonella enterica serovar Typhimurium. The G+C content of SPI-3 (47.5%) differs from that of the Salmonella genome (52%), consistent with the notion that these sequences have been horizontally acquired. SPI-3 harbors 10 open reading frames organized in six transcriptional units, which include the previously described mgtCB operon encoding the macrophage survival protein MgtC and the Mg2+ transporter MgtB. Among the newly identified open reading frames, one exhibits sequence similarity to the ToxR regulatory protein of Vibrio cholerae and one is similar to the AIDA-I adhesin of enteropathogenic Escherichia coli. The distribution of SPI-3 sequences varies among the salmonellae: the right end of the island, which harbors the virulence gene mgtC, is present in all eight subspecies of Salmonella; however, a four-gene cluster at the center of SPI-3 is found in only some of the subspecies and is bracketed by remnants of insertion sequences, suggesting a multistep process in the evolution of SPI-3 sequences.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Washington University School of Medicine, Department of Molecular Microbiology, 660 South Euclid Ave., Campus Box 8230, St. Louis, MO 63110. Phone: (314) 362-3692. Fax: (314) 362-1232. E-mail: groisman{at}borcim.wustl.edu.

Journal of Bacteriology, February 1999, p. 998-1004, Vol. 181, No. 3
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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