Previous Article | Next Article 
Journal of Bacteriology, February 1999, p. 1220-1228, Vol. 181, No. 4
Institute of Molecular Biology, National
Chung Hsing University, Taichung 40227, Taiwan, Republic of China
Received 10 September 1998/Accepted 8 December 1998
Thirty-two plasmid insertion mutants were independently isolated
from two strains of Xanthomonas campestris pv. campestris in Taiwan. Of the 32 mutants, 14 (44%), 8 (25%), and 4 (12%) mutants resulted from separate insertions of an IS3 family member,
IS476, and two new insertion sequences (IS),
IS1478 and IS1479. While IS1478
does not have significant sequence homology with any IS elements in the
EMBL/GenBank/DDBJ database, IS1479 demonstrated 73%
sequence homology with IS1051 in X. campestris
pv. dieffenbachiae, 62% homology with IS52 in
Pseudomonas syringae pv. glycinea, and 60% homology with
IS5 in Escherichia coli. Based on the predicted transposase sequences as well as the terminal nucleotide sequences, IS1478 by itself constitutes a new subfamily of the
widespread IS5 family, whereas IS1479, along
with IS1051, IS52, and IS5, belongs
to the IS5 subfamily of the IS5 family. All but
one of the IS476 insertions had duplications of 4 bp at the
target sites without sequence preference and were randomly distributed.
An IS476 insertion carried a duplication of 952 bp at the
target site. A model for generating these long direct repeats is
proposed. Insertions of IS1478 and IS1479, on
the other hand, were not random, and IS1478 and
IS1479 each showed conservation of PyPuNTTA and PyTAPu
sequences (Py is a pyrimidine, Pu is a purine, and N is any nucleotide)
for duplications at the target sites. The results of Southern blot
hybridization analysis indicated that multiple copies of
IS476, IS1478, and IS1479 are
present in the genomes of all seven X. campestris pv.
campestris strains tested and several X. campestris pathovars.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Characterization of Insertions of IS476
and Two Newly Identified Insertion Sequences, IS1478 and
IS1479, in Xanthomonas campestris pv.
campestris
*
Corresponding author. Mailing address: Institute of
Molecular Biology, National Chung Hsing University, Taichung, Taiwan
40227, Republic of China. Phone: 886-4-2851885. Fax: 886-4-2874879. E-mail: jhchen{at}dragon.nchu.edu.tw.
Journal of Bacteriology, February 1999, p. 1220-1228, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Lee, B.-M., Park, Y.-J., Park, D.-S., Kang, H.-W., Kim, J.-G., Song, E.-S., Park, I.-C., Yoon, U.-H., Hahn, J.-H., Koo, B.-S., Lee, G.-B., Kim, H., Park, H.-S., Yoon, K.-O., Kim, J.-H., Jung, C.-h., Koh, N.-H., Seo, J.-S., Go, S.-J.
(2005). The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice. Nucleic Acids Res
33: 577-586
[Abstract] [Full Text] -
Mamedov, I. Z., Lebedev, Y. B., Sverdlov, E. D.
(2004). Unusually long target site duplications flanking some of the long terminal repeats of human endogenous retrovirus K in the human genome. J. Gen. Virol.
85: 1485-1488
[Abstract] [Full Text] -
Lee, Y.-A., Fan, S.-C., Chiu, L.-Y., Hsia, K.-C.
(2001). Isolation of an Insertion Sequence from Ralstonia solanacearum Race 1 and Its Potential Use for Strain Characterization and Detection. Appl. Environ. Microbiol.
67: 3943-3950
[Abstract] [Full Text] -
Zhu, W., MaGbanua, M. M., White, F. F.
(2000). Identification of Two Novel hrp-Associated Genes in the hrp Gene Cluster of Xanthomonas oryzae pv. oryzae. J. Bacteriol.
182: 1844-1853
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»